Fig. 6

PF advanced the translocation of Nrf2 to the nucleus. HK-2 cells were treated with ML385 (5µM) for 24 h before PF pretreatment and H/R treatment. The grouping of blots cropped from different parts of the same gel. (A) The expression of cytoplasmic Nrf2 and HO-1 were measured by Western blotting. (B) Western blotting analysis of the expression of nuclear Nrf2. (C–E) The changes in the cytoplasmic Nrf2 and HO-1 protein expression were normalized to those of β-actin, and the change in the protein expression of nuclear Nrf2 was normalized to that of lamin B. The values are expressed as the mean ± SD (n = 3). *p < 0.05, ***p < 0.001, in comparison with the NC group. #p < 0.05, in comparison with the H/R group. ^p < 0.05, ^^^p < 0.001, in comparison with the H/R + PF group. NC, normal control, H/R, hypoxia/reoxygenation, PF, paeoniflorin, SD, standard deviation