Fig. 5

Effects of IL-33 and ST2 on ER stress and apoptosis in MGECs exposed to HG. (A) Western blots of MGECs showing the effect of IL-33 and ST2 exposed to HG. GAPDH was used as a loading control. Quantification relative to GADPH level. Results are representative of 1 in 3 replications. Data are means ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, **** p < 0.0001. (B) Flow cytometry analysis of apoptosis in MGECs. Representative images and apoptotic rates in cells show that a high concentration of IL-33 stimulation can reduce apoptosis. UR = late apoptotic cells; LR = early apoptotic cells; UR + LR = total apoptotic cells. The experiment was repeated at least three times. Data are means ± SEM. **p < 0.01, ***p < 0.001