Fig. 1
From: In vivo characterization of a podocyte-expressed short podocin isoform
PodocinΔexon5 mice lack exon 5 due to a deletion affecting the splice acceptor site. A Podocin isoform mRNA expression across different tissues in healthy human individuals. TPM = transcripts per million. Data Source: GTEx Analysis Release V8 (dbGaP Accession phs000424.v8.p2). B Schematic illustration of the podocin protein. Exon 5 is located within the characteristic PHB domain. C The CRISPR/Cas9 mediated deletion in podocinΔexon5 mice abolishes the splice acceptor site of exon 5 leading to an exon skipping in the final mRNA. ★ = site of deletion, 
= forward primer,
= reverse primer. Arrows indicate primers used for figure d. See Supplemental Fig. 1 for an amino acid alignment. D RT-PCR using the primers indicated in panel c. In podocinΔexon5/Δexon5 mice a piece of DNA representing exon 5 is missing. E Sanger sequencing of the cDNA of a podocin.Δexon5/Δexon5 mouse shows the in-frame transition from exon 4 directly to exon 6