Fig. 2
From: In vivo characterization of a podocyte-expressed short podocin isoform
PodocinΔexon5/Δexon5 mice display a severe congenital phenotype. A Not a single podocinΔexon5/Δexon5 mouse could be observed at the age of weaning (~ 3 weeks, n = 48 mice). B At birth (P0) the distribution of genotypes is as expected, according to Mendelian laws (n = 80 mice). The expected ratio of observed genotypes was confirmed using a Chi-Square test. C Colloidal coomassie staining of urine samples from podocinwildtype/wildtype, podocinΔexon5/wildtype and podocinΔexon5/Δexon5 mice at P0. As compared to the other two genotypes, more albumin could be detected in urine of podocinΔexon5/Δexon5 mice. Full-length gel is presented in Suppl. Figure 4. D Quantifications of the urinary albumin creatinine ratio of podocinwildtype/wildtype, podocinΔexon5/wildtype and podocinΔexon5/Δexon5 mice at P0. The ratio was significantly higher in podocinΔexon5/Δexon5 mice. Tukey’s multiple comparison test was used to determine statistical significance. Data are presented as mean ± SEM (n ≥ 3 mice per genotype). * p < 0.05. E PAS staining of podocinwildtype/wildtype, podocinΔexon5/wildtype and podocinΔexon5/Δexon5 mice at P0. No sclerotic lesions could be found, however multiple proteins casts were detectable in the tubular system of PodocinΔexon5/Δexon5 mice (asterisk). Scale bars correspond to 200 µm and 40 µm (insets), respectively. F Wt-1 Immunohistochemistry of podocinwildtype/wildtype, podocinΔexon5/wildtype and podocinΔexon5/Δexon5 mice at P0. Distribution of Wt-1 positive cells was not globally altered among the three genotypes. Scale bars correspond to 200 µm