Fig. 3
From: In vivo characterization of a podocyte-expressed short podocin isoform
Quantifications of Nphs1 and Nphs2 mRNA and podocin and nephrin protein in kidneys of podocinwildtype/wildtype, podocinΔexon5/wildtype and podocinΔexon5/Δexon5 mice at P0. A Overall Nphs2 mRNA levels were not significantly different among the three genotypes. B A custom designed qPCR assay specifically quantifies the Nphs2Δexon5 mRNA. C Using a targeted mass spectrometry approach, podocin abundance was measured in podocinwildtype/wildtype, podocinΔexon5/wildtype and podocinΔexon5/Δexon5 mice at P0. Podocin levels were significantly lower in podocinΔexon5/wildtype and podocinΔexon5/Δexon5 mice. D Using a targeted mass spectrometry approach, nephrin abundance was measured in podocinwildtype/wildtype, podocinΔexon5/wildtype and podocinΔexon5/Δexon5 mice at P0. Nephrin levels were significantly lower in podocinΔexon5/wildtype and podocinΔexon5/Δexon5 mice. E Quantification of Nphs1 mRNA in kidneys of podocinwildtype/wildtype, podocinΔexon5/wildtype and podocin.Δexon5/Δexon5 mice at P0. Tukey’s multiple comparison tests were used to determine statistical significance. Data are presented as mean ± SEM (n = 4 mice per genotype, each circle/square/triangle represents one mouse). * p < 0.05, ** p < 0.01, *** p < 0.001