Fig. 4
From: In vivo characterization of a podocyte-expressed short podocin isoform
STED imaging reveals disrupted foot process morphology in podocinΔexon5/Δexon5 mice. A-C STED imaging following immunolabelling with antibodies against nephrin and podocin in samples of podocinwildtype/wildtype (A), podocinΔexon5/wildtype (B) and podocinΔexon5/Δexon5 (C) mice at P0. Scale bars correspond to 5 µm. (D)-(E) Conventional immunofluorescence microscopy of HeLa cells transiently transfected with FLAG-tagged podocinwildtype (D) and podocinΔexon5 (E) confirms the retaining of podocinΔexon5 in the endoplasmic reticulum. Scale bars correspond to 20 µm