Fig. 2

Effect of iron administration on the log-transformed Fe content (A), the relative mRNA levels of ferritin H (B) and Nrf2 (C) in the aortas of the adenine-treated rats fed a high-phosphorus diet, and the correlation between the relative liver mRNA level of HAMP and log-transformed aortic Fe content (R = 0.82, p = 0.0003) (D), between the log-transformed aortic Fe content and relative ferritin H expression in the aorta (R = 0.65, p = 0.012) (E), between the log-transformed aortic Fe content and the aortic mRNA level of Nrf2 (R = 0.71, p = 0.004) (F), and between the aortic mRNA level of Nrf2 and aortic mRNA level of ferritin H (R = 0.89, p = 0.00003) (G). The No-Fe group, PO-Fe group and IP-Fe group were CKD rats without Fe administration, with peroral Fe administration and with intraperitoneal Fe administration, respectively. Data are expressed as the mean ± SD and were compared by one-way analysis of variance followed by the Tukey–Kramer test. Linear regression analyses were used to determine the correlations between parameters. A two-tailed p < 0.05 was considered statistically significant