Table 1 Primer sequences used in the qRT‒PCR analysis
Gene | Forward | Reverse |
|---|---|---|
HAMP | 5’-CTGAGCAGCGGTGCCTATCT-3’ | 5’-GCACTGTCATCAGTCTTGCTTTC-3’ |
Nrf2 | 5’- GCTGCCATTAGTCAGTCGCTCTC-3’ | 5’-ACCGTGCCTTCAGTGTGCTTC-3’ |
Ferritin H | 5’- ACCAGCGAGGTGGACGAATC-3’ | 5’-ATTCAGGTAATGCGTCTCAATGAAG-3’ |
HIF-1α | 5’- GCTACAAGAAACCGCCTA-3’ | 5’-GTTCTTCTGGCTCATAACCC-3’ |
RUNX2 | 5’- CACAGGGTGACTCCCGTTACAA-3’ | 5’- TGTGACCCAGTGCAAATGAAGA-3’ |
Hmox1 | 5’-ATTTGTCCGAGGCCTTGAA-3’ | 5’-CCAGGGCCGTATAGATATGGTA-3’ |
Nqo1 | 5’-TGAGCCCGGATATTGTAGCTGA-3’ | 5’-GCATACGTGTAGGCGAATCCTG-3’ |
β-actin | 5’- GGAGATTACTGCCCTGGCTCCTA-3’ | 5’-GACTCATCGTACTCCTGCTTGCTG-3’ |