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Fig. 5 | BMC Nephrology

Fig. 5

From: Assessing the effects of tempol on renal fibrosis, inflammation, and oxidative stress in a high-salt diet combined with 5/6 nephrectomy rat model: utilizing oxidized albumin as a biomarker

Fig. 5

Western blot testing. (a)Representative western blots of Smad3/GAPDH, TGF-β1/GAPDH. (b)Quantitative analysis of Smad3. (c)Quantitative analysis of TGF-β1. Compared to the Blank group, Smad3 protein and TGF-β1 protein expression in the other three groups were elevated, with the highest expression in the high-salt diet group followed by the treatment group, and the normal diet group had lower expression than the treatment group. (d)Representative western blots of P65-NF-κB/GAPDH. (e)Quantitative analysis of P65-NF-κB. Compared with the Blank group, the expression of p65-NF-κB protein was increased in the remaining three groups, with the highest expression in high salt diet group, followed by the treatment group, and the Normal diet group was lower than the treatment group. (f) Quantitative analysis of Collagen I. (g)Representative western blots of Collagen I/GAPDH. Compared to the Blank group, the expression of Collagen I protein was increased in the other three groups, with the highest expression in the high salt diet group, treatment with Tempol significantly ameliorated Collagen I protein expression in high-salt diet group rats although still higher than normal-salt diet group rats. The values are shown as AVE ± SEM

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BMC Nephrology

ISSN: 1471-2369

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