Fig. 1

The expression of circ-Gatad1 was increased in septic acute kidney. (A) High-throughput sequencing analysis identified a series of upregulated and downregulated circRNAs between healthy and AKI. (B) RT-qPCR detection show that the expression of circ-Gatad1 was increased in septic acute kidney patients. Data are expressed as mean ± SD. ^***p < 0.001 vs. Healthy. (C) RT-qPCR detection show that the expression of circ-Gatad1 was increased in HK2 cells after treatment with different dose of LPS (0–15 µg/ml). Data are expressed as mean ± SD. ^***p < 0.001 vs. 0 µg/ml. (D and E) Representative FISH images and subcellular fractionation assays showed the subcellular distribution of circ-Gatad1 in HK2 cells. U6 was used as a nuclear control and 18 S rRNA was used as a cytoplasmic control. Scales bar, 20 μm. (F) After RNase R treatment, the levels of circ-Gatad1 and linear Gatad1 mRNA were determined using qRT-PCR. Data are expressed as mean ± SD. ^***p < 0.001 vs. Mock