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Fig. 2 | BMC Nephrology

Fig. 2

From: Knockdown of circ-Gatad1 alleviates LPS induced HK2 cell injury via targeting miR-22-3p/TRPM7 axis in septic acute kidney

Fig. 2

Downregulation circ-Gatad1 suppressed LPS-treated induced HK2 cells injury. (A) RT-qPCR detection show that the expression of circ-Gatad1 in HK2 cells after transfected with siRNA against circ-Gatad1. Data are expressed as mean ± SD. ***p < 0.001 vs. NC. ###p < 0.001 vs. LPS. (B) CCK8 detection show the cells proliferation ability in HK2 cells. Data are expressed as mean ± SD. ***p < 0.001 vs. NC. ###p < 0.001 vs. LPS. (C and D) Flow apoptosis detection show the apoptosis of HK2 cells. Data are expressed as mean ± SD. ***p < 0.001 vs. NC. ###p < 0.001 vs. LPS. (E and F) Dihydroethidium (DHE) staining show the ROS level in HK2 cells. Data are expressed as mean ± SD. **p < 0.01, ***p < 0.001 vs. NC. ###p < 0.001 vs. LPS. Scales bar, 50 μm. (G-I) ELISA detection show the expression of inflammatory factor TNF-α, IL-1β, IL-6. Data are expressed as mean ± SD. Data are expressed as mean ± SD. *p < 0.05, ***p < 0.001 vs. NC. ##p < 0.01, ###p < 0.001 vs. LPS

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BMC Nephrology

ISSN: 1471-2369

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