Fig. 3

miR-22-3p and TRPM7 were downstream targets of circ-Gatad1. (A) Bioinformatics analysis predicting binding sites of miR-22-3p in circ-Gatad1. Mutant version of circ-Gatad1 is shown. (B) Relative luciferase activity determined 48 h after transfection of HK2 cells with miR-22-3p mimic/NC or circ-Gatad1 wild-type/Mut. Data are means ± SD. ***P < 0.001. (C) Prediction of miR-22-3p binding sites in the 3’-UTR of TRPM7. Mutant version of 3’-UTR-TRPM7 is shown. (D) Relative luciferase activity 48 h after transfection of HK2 cells with miR-22-3p mimic/NC or 3’-UTR-TRPM7 wild-type/Mut. Data are means ± SD. **P < 0.01. (E-G) RT-qPCR detection show the expression of circ-Gatad1, miR-22-3p and TRPM7 in HK2 cells. Data are expressed as mean ± SD. ^**p < 0.01, ^***p < 0.001 vs. NC. ^##p < 0.01, ^###p < 0.001 vs. si-circ-Gatad1