Fig. 4

Overexpression of TRPM7 or downregulation of miR-22-3p reversed the protective effect of si-circ-Gatad1 to HK2 after exposure to LPS (5 µg/ml) microenvironment. (A) CCK8 detection show the cells proliferation ability in HK2 cells. Data are expressed as mean ± SD. ^***p < 0.001 vs. NC. ^###p < 0.001 vs. si-circ-Gatad1. (B and C) Flow apoptosis detection show the apoptosis of HK2 cells. Data are expressed as mean ± SD. ^***p < 0.001 vs. NC. ^###p < 0.001 vs. si-circ-Gatad1. (D and E) Dihydroethidium (DHE) staining show the ROS level in HK2 cells. Data are expressed as mean ± SD. ^***p < 0.001 vs. NC. ^###p < 0.001 vs. si-circ-Gatad1. Scales bar, 50 μm. (F-H) ELISA detection show the expression of inflammatory factor TNF-α, IL-1β, IL-6. Data are expressed as mean ± SD. Data are expressed as mean ± SD. ^***p < 0.001 vs. NC. ^###p < 0.001 vs. si-circ-Gatad1