Fig. 2

Renal histopathology at various developmental stages of C57BL/6 mice. (a) The four basic stains (HE, PAS, PASM, Masson) of kidney pathology in the indicated mouse groups. Scale bars, 25 μm (b, c) Glomerular tuft area and glomerular tuft volume of each group. n = 3 mice per group*7–9 glomerular per mice. (d) Quantification of the mesangial matrix fraction. n = 3 mice per group*4–6 glomerular per mice. (e) Quantification of the fibrosis area. n =  3 mice per group*4–6 glomerular per mice. (f) Immunofluorescence staining for IgG and IgM in kidney sections from different life stages of mice. Scale bar = 50 μm. Typical images are shown. Calculation of glomerular area and volume and quantification of mesangial expansion was based on glomeruli cut at the vascular pole per mouse in each group. Data are expressed as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. (g) Representative Masson images