Fig. 3

Podocyte characteristics and GBM changes with age in mice. (a) WT1 and synapotopodin in kidney sections from the indicated mouse groups. Scale bar = 20 μm. (b) Display of knobs-like structure under light microscope (PAS, PASM staining) and electron microscope. Scale bar of light microscope = 10 μm. Scale bar of electron microscope = 10 μm. (c) Quantification of WT1 + podocytes per glomerular cross-section. (d) Quantification of foot process width. (e) Quantification of GBM thickness. (f) Quantitative statistics and analysis of knobs-like structures in each group. (g) Correlation analysis between number of knobs-like structures and Scr. (h) Correlation analysis between number of knobs-like structures and Bun. (i) Correlation analysis between number of knobs-like structures and UACR. 3 mice per group. Data are expressed as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001