Fig. 4

Single-cell transcriptome analysis reveals differences in gene levels with age in mice. (a) soft k-means clustering analysis of whole kidney cells identifies 14 cell clusters corresponding to 12 different cell types. (b) Differentially expressed genes in different cell types. (c,d) GO and KEGG enrichment analysis of genes within senescent mice compared with maturing mice. (e,f,g) Respectively represent the normalized expression of Lamc1, Lama3 and Agrn gene in middle-age and senescence. (h) Single cell-type specific average expression of human monogenic disease genes. Mean expression values of the genes were calculated in each cluster. In the heatmap, each row represents one gene and each column is single cell type