Fig. 5
From: Circular RNA hsa_circ_0005519 contributes to acute kidney injury via sponging microRNA-98-5p
Effects of miR-98-5p inhibitor on cell viability, inflammatory response and oxidative stress regulated by hsa_circ_0005519 in LPS-treated HK-2 cells. (A) Reverse transcription-qPCR analysis of miR-98-5p after LPS stimulation. (B) Reverse transcription-qPCR analysis of miR-98-5p after transfection or co-transfection in LPS-treated HK-2 cells. (C) The changes in cell viability were determined using MTT assay. (D) The concentration of IL-6, TNF-α, and IL-1β were measured by ELISA. The MPO activity (E) and MDA (F) content were detected using commercialized detection kits. Statistical analysis comparing two groups was by Mann-Whitney U test. ***p < 0.001, vs. in-NC. ##p < 0.01, ###p < 0.001,vs. LPS + anti-NC. &&p < 0.01, &&&p < 0.001,vs. anti-CIRC + in-miR-98-5p. LPS, lipopolysaccharide; MPO, myeloperoxidase; MDA, malondialdehyde; ELISA, enzyme-linked immunosorbent assay