Fig. 2

Silencing of differentiation antagonizing non-protein coding ribonucleic acid (DANCR) alleviates high glucose (HG)-induced renal tubular epithelial cell injury. A. The DANCR levels gradually increased with prolonged HG induction. B. In HG-induced human kidney 2 (HK-2) transfection with small interfering (si)-DANCR, a quantitative reverse transcription polymerase chain reaction was performed to investigate DANCR levels. HG-induced and si-DANCR-transfected HK-2 cell proliferation and apoptosis were detected using the Cell Counting Kit-8 (C) and flow cytometry (D). Enzyme-linked immunosorbent assay kits and commercial kits were used to evaluate the secretion of inflammatory factors (E), reactive oxygen species production (F), and malondialdehyde content (G). *** P < 0.001 vs. control; ### P < 0.001 vs. HG + si-negative control