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Correlation of histopathologic and dynamic tissue perfusion measurement findings in transplanted kidneys
© Scholbach et al.; licensee BioMed Central Ltd. 2013
Received: 19 November 2012
Accepted: 27 June 2013
Published: 11 July 2013
Cortical perfusion of the renal transplant can be non-invasively assessed by color Doppler ultrasonography. We performed the Dynamic Tissue Perfusion Measurement (DTPM) of the transplant’s renal cortex using color Doppler ultrasonography (PixelFlux technique), and compared the results with the histopathological findings of transplant biopsies.
Ninety-six DTPM studies of the renal transplant’s cortex followed by transplant biopsies were performed in 78 patients. The cortical perfusion data were compared with the parameter of peritubular inflammatory cell accumulation (PTC 0 to 3) based on Banff-classification system.
A significant decrease of cortical perfusion could be demonstrated as the inflammatory cells accumulation in peritubular capillaries increased. Increasing peritubulitis caused a perfusion loss from central to distal layers of 79% in PTC 0, of 85% in PTC 1, of 94% in PTC 2, and of 94% in PTC 3. Furthermore, the perfusion loss due to peritubular inflammation was more prominent in the distal cortical layer. The extent of perfusion decline with increasing peritubulitis (from PTC 0 to PTC 3) was 64% in proximal 20% cortical layer (p20), 63% in proximal 50% cortical layer (p50), increased to 76% in distal 50% cortical layer (d50), and peaked at 90% in the distal 20% cortical layer (d20). For those without peritubulitis (PTC 0), the increase in the the Interstitial Fibrosis/Tubular Atrophy (IF/TA) score was accompanied by a significantly increased cortical perfusion. A Polyomavirus infection was associated with an increased cortical perfusion.
Our study demonstrated that the perfusion of the renal transplant is associated with certain pathological changes within the graft. DTPM showed a significant reduction of cortical perfusion in the transplant renal cortex related to peritubular capillary inflammation.
Acute and chronic damage to renal transplants requires an early and accurate diagnosis to start the appropriate treatment. Renal transplant biopsies are an established method to evaluate such changes. These biopsies are performed as routine protocol biopsies in regular intervals in asymptomatic recipients or are performed when a clinical demand emerges. Biopsies are invasive and therefore a need for less potentially harmful procedures exists. Sonography is widely used to describe morphological changes of the transplant. Doppler sonography is used to describe changes of the blood flow to transplants. Today, only limited information can be drawn from Doppler sonographic measurements. To refine the impression of a transplant’s perfusion contrast enhanced sonography was introduced. Nevertheless, a need for a more differentiated evaluation remains. Dynamic tissue perfusion measurement (DTPM) has demonstrated the potential to describe perfusion in renal cortex in thin cortical slices with more than 50 parameters [1–5] as well as in other organs [6–10].
Our aim was to compare histopathological descriptions of renal transplants with DTPM data to plumb the potential of this new tool.
This study was approved by Institutional Review Board. Totally, 96 DTPM studies and renal transplant biopsies were performed in 78 Taiwanese patients (single study for 63 patients; two studies for 12 patients; three studies for 3 patients) between December 2005 and September 2008. The mean duration between the patient receiving renal transplantation and DTPM study was 44.8 months. Among 78 patients, there were 41 male and 37 female; the mean age when receiving living or deceased donors’ renal transplantation was 41.0 ± 12.6 years (range 6–72 years); 68 received renal transplantation from deceased donor, 6 received simultaneous pancreas and kidney transplantation, 4 received living-related renal transplantation. The indication for grafting was end stage renal disease; the transplant surgery was performed in Taiwan or People’s Republic of China. Details of the transplant recipients are outlined below:
Informed consent was obtained from every patient before the study.
This study was supported by research grants 96-2321-B-075-009 and 97-2314-B-075-052 from the National Science Council, Taiwan and was approved by the Institutional Review Board, Taipei Veterans Genereal Hospital, Taipei, Taiwan.
Color doppler sonography and dynamic tissue perfusion measurement
Workflow chart for Dynamic Tissue Perfusion Measurement (DTPM) of transplant kidneys
The software opened the video and calibrated the distances automatically
The software read out the color bar and all hues were calibrated
Maximal velocities encoded by the colors were registered
Selection of the region of interest (ROI)
a. The ROI consisted of a parallelogram which included an entire cortical segment in the center of the transplant, fed by one interlobar artery running straight towards the transducer.
b. The four corners of the parallelogram were set as follows:
i. At the center of the outer edge of a medullary pyramid (1)
ii. At the center of the outer edge of the neighboring medullary pyramid (2)
iii. At the renal surface perpendicular to the first two corners (3 and 4)
c. The ROI was sliced into defined horizontal slices
i. Each slice stretched from the left to the right border of the ROI and had a height which encompassed a certain percentage of the ROI’s entire height (10%, 20% , 50% or 100%). So the sub-ROI encompassing the proximal 20% (resp. 50%) was labeled p20 (resp. p50) and those encompassing the distal 20% (resp. 50%) was labeled d20 (resp. d50). (Figures 1 and 2)
ii. These slices were arranged to cover adjacent horizontal strips of the ROI.
Dynamic tissue perfusion measurement was initiated. All steps (a-f) were carried out automatically by the PixelFlux-software
a. Each pixel (colored or grey) was evaluated with respect to its size and coloration.
b. The color bar’s hues were assigned a velocity value according to its relative distance from zero to the maximum velocity value.
c. The relative distance value (from 0 to 1) was then multiplied by the maximum velocity indicated to calculate a specific velocity value for each color hue. Colorless pixels were assigned the velocity of zero.
d. The mean velocity value of all colored pixels inside the ROI and the area of all colored pixels were calculated.
e. The measurements were repeated for all consecutive images of the video.
f. The software recognized beginning and end of any heart cycle inside the video automatically and restricted all consecutive calculations to a full heart cycle.
g. Then the mean perfusion velocity of each image was multiplied by the mean perfused area. This product was divided by the area of the entire ROI resulting in the mean flow intensity of the ROI during a complete heart cycle.
Transplant biopsies were performed under sonographic-guidance by a single investigator (H.-K. W). An 18-gauge core biopsy needle (Temno, Cardinal Health, McGaw Park, IL, USA) was used to sample tissue from the outer cortex including parts of the cortico-medullary junction of the transplanted kidney. The specimens were formalin-fixed for light microscopy; glutaraldehyde-fixed for electron microscopy; or placed in phosphate buffered solution for immunofluorescent microscopy.
Banff classification of inflammatory cells in peritubular capillaries (PTCs), degree of interstitial fibrosis (IF/TA), and stages of polyomavirus infection (BKN)
No significant cortical peritubular inflammatory changes
Cortical peritubular capillary with 3–4 luminal inflammatory cells
Cortical peritubular capillary with 5–10 luminal inflammatory cells
Cortical peritubular capillary with >10 luminal inflammatory cells
No interstitial fibrosis and tubular atrophy
Mild interstitial fibrosis and tubular atrophy (<25% of cortical area)
Moderate interstitial fibrosis and tubular atrophy (26–50% of cortical area)
Severe interstitial fibrosis and tubular atrophy/ loss (>50% of cortical area)
BKN- stage A
Viral activation in cortex and/or medulla with intranuclear inclusion bodies AND/OR positive immunohistochemistry or in situ hybridization signals, no or minimal tubular epithelial cell necrosis/lysis, no denudation of tubular basement membranes, no or minimal interstitial inflammation in foci with viral activation, no or minimal tubular atrophy and interstitial fibrosis
BKN- stage B
Marked viral activation in cortex and/or medulla, marked virally induced tubular epithelial cell necrosis/lysis and associated denudation of tubular basement membranes, interstitial inflammation, interstitial fibrosis and tubular atrophy
Data were explored statistically using SPSS 17 and PASW 18 software. Groups were compared applying the Mann–Whitney-U-test. P-values less than 0.05 were regarded statistically significant.
Among 96 DTPM studies, 36 belonged to the PTC 0-group, 46 to the PTC 1-group, 11 to the PTC 2-group and 3 to the PTC 3-group; 47 belonged to the IF/TA 0-group, 32 to the IF/TA 1-group, 17 to the IF/TA 2-group and 0 to the IF/TA 3-group.
Mean values, numbers (N) and standard deviations (SD) of global cortical perfusion intensity in DTPM and resistance indices (RI) in groups with different PTC scores
Perfusion intensity [cm/s]
p-values of Mann–Whitney-U-tests for differences of global cortical perfusion intensity in DTPM between different PTC scores
Perfusion intensity [cm/s]: differences between PTC groups
Renal transplant biopsies are evaluated according to the Banff scoring system since it was shown that a high Banff score is predictive for the graft survival .
Color Doppler ultrasound of the outer renal cortex provides the basis for a quantitative measurement of the perfusion of renal transplants [1, 2, 14]. Vascular changes, which are mirrored by perfusion changes, may precede manifest function loss of the kidney and can be described histologically.
Peritubular inflammation is a criterion of humoral rejection in renal transplants. According to the Banff classification, this feature is scored as peritubular capillaritis (PTCs) . It might be important to detect peritubular capillaritis in renal transplants early since it can precede the development of a full blown chronic antibody-mediated rejection (CAMR). Others propose to assume a chronic antibody-mediated rejection (CAMR) even in the absence of C4d deposition in cases with peritubular capillaritis, transplant glomerulitis, thickening of the peritubular capillaries basement membrane, and circulating anti-HLA antibodies [16, 17]. Detection of peritubular inflammation might be significant for indicating the subsequent poor prognosis [18, 19] and thus could be regarded an early sign of graft deterioration. Our study showed a significant decrease of cortical perfusion as the inflammatory cells accumulation in peritubular capillaries increased. The reduction of tissue perfusion due to peritubular inflammation depended on the position of the cortical layer too. The more distal the layer the stronger was the effect of peritubular inflammation. Cortical perfusion was also influenced by interstitial fibrosis and tubular atrophy (IF/TA). Unexpectedly, increasing IF/TA scores were connected to an increased perfusion. This might be explained by the short-circuiting of blood via shunts connecting the afferent and efferent vascular pools in juxtamedullary glomeruli [20, 21]. Recently, in a mouse model the overexpression of EphB4 receptor tyrosine kinase in the kidney was shown to promote the development of such shunts .
Conventional color Doppler sonography with calculation of Resistance Index (RI) and Pulsatility Index (PI) were used for many years to evaluate the perfusion of renal transplants. RI measurements reflect a complex interaction of renal as well as systemic vascular influences and are therefore disregarded to be suitable for evaluation of the renal vascular state by some investigators [23, 24]. An elevated RI alone cannot reliably differentiate the causes of transplanted kidney dysfunction and it is considered a nonspecific marker .
RI does not inform about the real blood flow velocities, since a ratio of two velocities (systolic and diastolic) is calculated. It represents spatially restricted information from a single vessel and gives no data on the width of the vessel, let alone the perfused area in a certain tissue. Thus RI is basically less suitable for describing the perfusion status of the renal parenchyma. Furthermore, the pathological changes inside the kidney can be distributed inhomogeneously , and RI at a single point of an artery may not represent the status of perfusion in the region of interest. In contrast, Dynamic Tissue Perfusion Measurement (DTPM) is based upon a heart cycle triggered measurement of a tissue’s mean flow velocity of all vessels inside the region of interest multiplied by the mean perfused area (respecting changing width of all vessels during the heart cycle) [3, 4]. Thus, DTPM offers a more sophisticated investigation of renal perfusion by using velocity as well as perfused area information, referring all measurements to all vessels inside the tissue and is capable in differentiating all perfusion data between millimeter-thin slices. It reflects histological changes as PTCs better than conventional RI (Figure 3: left diagram compared to the right one). DTPM thus has the potential to go beyond the limitations of usual vascular resistance calculations by RI measurements. The detection of subtle perfusion changes simultaneously to a polyomavirus infection (Figure 6) clearly demonstrates that the slice wise perfusion measurement in consecutive layers of renal microvasculature offers a sophisticated access to relevant histological changes even beyond the Banff-classification. It was demonstrated in earlier investigations that with time elapsed after transplantation the cortical perfusion in renal transplants of children decreased significantly  and that simultaneously the pulsatility of renal transplants’ microvascular perfusion increased significantly . Reduction of smaller subcapsular transplant vessels occurs in acute as well as chronic rejected organs . Cortical vessel loss, as determined visually in power Doppler images, was predictive in contrary to RI as for the transplant function 1 year after transplantation . Recent investigations also showed that microvascular damage is the key process linked to the loss of transplants’ function [29, 30]. Our findings might be especially noteworthy since we demonstrated a significant and gradual decline of cortical perfusion along with increasing peritubular capillaritis using DTPM. DTPM clearly showed the potential to measure the progressive obliteration of the transplant’s microvessels. This process starts in the periphery of renal cortex where the vessel diameters are naturally small. Our study did not investigate the relationship of DTPM with the clinical outcome of the graft. Further studies are needed to elucidate if specific histological findings or the destruction of the microvasculature are more predictive for the transplant’s function.
One limitation of our study is that we only assess the relationship between cortical perfusion and the histopathological changes of transplant kidney. Future studies are needed to confirm the role of DTPM as a surrogate of outcome of renal transplant. Then DTPM could become a non-invasive tool to reduce the need for invasive procedures such as biopsies.
Microvascular perfusion changes in renal transplants can be demonstrated by DTPM. A significant reduction of cortical perfusion in the transplant kidney, especially in its peripheral layers, is related to peritubular capillary inflammation.
This study was supported by research grants 96-2321-B-075-009 and 97-2314-B-075-052 from the National Science Council, Taiwan.
- Scholbach T, Dimos I, Scholbach J: A new method of color Doppler perfusion measurement via dynamic sonographic signal quantification in renal parenchyma. Nephron Physiol. 2004, 96 (4): p99-p104. 10.1159/000077380.View ArticlePubMedGoogle Scholar
- Scholbach T, Girelli E, Scholbach J: Dynamic tissue perfusion measurement: a novel tool in follow-up of renal transplants. Transplantation. 2005, 79 (12): 1711-1716. 10.1097/01.TP.0000164145.89275.02.View ArticlePubMedGoogle Scholar
- Scholbach T, Scholbach J: Can we measure renal tissue perfusion by ultrasound?. J Med Ultrasound. 2009, 17 (1): 9-16. 10.1016/S0929-6441(09)60010-2.View ArticleGoogle Scholar
- Scholbach T, Scholbach J: Dynamic sonographic tissue perfusion measurement. J Med Ultrasound. 2009, 17 (2): 71-85. 10.1016/S0929-6441(09)60114-4.View ArticleGoogle Scholar
- Scholbach T: From the nutcracker-phenomenon of the left renal vein to the midline congestion syndrome as a cause of migraine, headache, back and abdominal pain and functional disorders of pelvic organs. Med Hypotheses. 2007, 68 (6): 1318-1327. 10.1016/j.mehy.2006.10.040.View ArticlePubMedGoogle Scholar
- Wieczorek AP, Wozniak MM, Stankiewicz A, Bogusiewicz M, Santoro GA, Rechberger T, Scholbach J: The assessment of normal female urethral vascularity with Color Doppler endovaginal ultrasonography: preliminary report. Pelviperineology. 2009, 28: 59-61.Google Scholar
- Scholbach T, Herrero I, Scholbach J: Dynamic color Doppler sonography of intestinal wall in patients with Crohn disease compared with healthy subjects. J Pediatr Gastroenterol Nutr. 2004, 39 (5): 524-528. 10.1097/00005176-200411000-00014.View ArticlePubMedGoogle Scholar
- Scholbach T, Scholbach J, Krombach GA, Gagel B, Maneschi P, Di Martino E: New method of dynamic color doppler signal quantification in metastatic lymph nodes compared to direct polarographic measurements of tissue oxygenation. Int J Cancer. 2005, 114 (6): 957-962. 10.1002/ijc.20827.View ArticlePubMedGoogle Scholar
- Gagel B, Piroth M, Pinkawa M, Reinartz P, Zimny M, Kaiser HJ, Stanzel S, Asadpour B, Demirel C, Hamacher K, et al: pO polarography, contrast enhanced color duplex sonography (CDS), [18F] fluoromisonidazole and [18F] fluorodeoxyglucose positron emission tomography: validated methods for the evaluation of therapy-relevant tumor oxygenation or only bricks in the puzzle of tumor hypoxia?. BMC Cancer. 2007, 7: 113-10.1186/1471-2407-7-113.View ArticlePubMedPubMed CentralGoogle Scholar
- Scholbach T, Hormann J, Scholbach J: Dynamic tissue perfusion measurement of the intestinal wall - correlation to histology in ulcerative colitis. J Med Ultrasound. 2010, 18 (2): 62-70. 10.1016/S0929-6441(10)60009-4.View ArticleGoogle Scholar
- Racusen LC, Solez K, Colvin RB, Bonsib SM, Castro MC, Cavallo T, Croker BP, Demetris AJ, Drachenberg CB, Fogo AB, et al: The Banff 97 working classification of renal allograft pathology. Kidney Int. 1999, 55 (2): 713-723. 10.1046/j.1523-1755.1999.00299.x.View ArticlePubMedGoogle Scholar
- Racusen LC, Halloran PF, Solez K: Banff 2003 meeting report: new diagnostic insights and standards. Am J Transplant. 2004, 4 (10): 1562-1566. 10.1111/j.1600-6143.2004.00585.x.View ArticlePubMedGoogle Scholar
- Kaminska D, Bernat B, Mazanowska O, Krasnowski R, Polak W, Patrzalek D, Kochman A, Klinger M: Predictive value of Banff score of early kidney allograft biopsies for 1-year graft survival. Transplant Proc. 2006, 38 (1): 59-61. 10.1016/j.transproceed.2005.12.027.View ArticlePubMedGoogle Scholar
- Scholbach T, Girelli E, Scholbach J: Tissue pulsatility index: a new parameter to evaluate renal transplant perfusion. Transplantation. 2006, 81 (5): 751-755. 10.1097/01.tp.0000201928.04266.d2.View ArticlePubMedGoogle Scholar
- Bhowmik DM, Dinda AK, Mahanta P, Agarwal SK: The evolution of the Banff classification schema for diagnosing renal allograft rejection and its implications for clinicians. Indian J Nephrol. 2010, 20 (1): 2-8. 10.4103/0971-4065.62086.View ArticlePubMedPubMed CentralGoogle Scholar
- Shimizu T, Ishida H, Shirakawa H, Omoto K, Tsunoyama K, Iida S, Tanabe K, Yamaguchi Y: Clinicopathological analysis of transplant glomerulopathy cases. Clin Transplant. 2009, 23 (Suppl 20): 39-43.View ArticlePubMedGoogle Scholar
- Masutani K, Kitada H, Noguchi H, Tsuruya K, Katafuchi R, Tanaka M, Iida M: Subclinical peritubular capillaritis in serial graft biopsies in cadaveric kidney transplant recipient with pre-transplant anti-HLA antibodies. Clin Transplant. 2009, 23 (Suppl 20): 34-38.View ArticlePubMedGoogle Scholar
- Wang R, Wang H, Chen J, Wu J, Wang Y, Huang H, He Q: C4d deposition in allograft renal biopsies is an independent risk factor for graft failure. Nephrology (Carlton). 2009, 14 (5): 527-532. 10.1111/j.1440-1797.2009.01095.x.View ArticleGoogle Scholar
- Kieran N, Wang X, Perkins J, Davis C, Kendrick E, Bakthavatsalam R, Dunbar N, Warner P, Nelson K, Smith KD, et al: Combination of peritubular c4d and transplant glomerulopathy predicts late renal allograft failure. J Am Soc Nephrol. 2009, 20 (10): 2260-2268. 10.1681/ASN.2009020199.View ArticlePubMedPubMed CentralGoogle Scholar
- Trueta J, Barclay AE, Darmady EM, Paramore RH, Himsworth HP: Discussion on renal circulation. Proc R Soc Med. 1948, May, 41 (5): 339-342.PubMedGoogle Scholar
- Lynch M: Some observations on glomerular vascular architecture. Can Med Assoc J. 1957, Oct 1, 77 (7): 679-686.PubMedPubMed CentralGoogle Scholar
- Andres A, Munarini N, Djonov V, Bruneau S, Zuercher G, Loercher S, Rohrbach V, Ziemiecki A: EphB4 receptor tyrosine kinase transgenic mice develop glomerulopathies reminiscent of aglomerular vascular shunts. Mech Dev. 2003, 120 (4): 511-516. 10.1016/S0925-4773(02)00461-6.View ArticlePubMedGoogle Scholar
- Heine GH, Reichart B, Ulrich C, Kohler H, Girndt M: Do ultrasound renal resistance indices reflect systemic rather than renal vascular damage in chronic kidney disease?. Nephrol Dial Transplant. 2007, 22 (1): 163-170.View ArticlePubMedGoogle Scholar
- Gerhart MK, Seiler S, Grun OS, Rogacev KS, Fliser D, Heine GH: Indices of systemic atherosclerosis are superior to ultrasound resistance indices for prediction of allograft survival. Nephrol Dial Transplant. 2010, 25 (4): 1294-1300. 10.1093/ndt/gfp631.View ArticlePubMedGoogle Scholar
- Kocabas B, Aktas A, Aras M, Isiklar I, Gencoglu A: Renal scintigraphy findings in allograft recipients with increased resistance index on Doppler sonography. Transplant Proc. 2008, 40 (1): 100-103. 10.1016/j.transproceed.2007.11.004.View ArticlePubMedGoogle Scholar
- Nadasdy T, Allen C, Zand MS: Zonal distribution of glomerular collapse in renal allografts: possible role of vascular changes. Hum Pathol. 2002, 33 (4): 437-441. 10.1053/hupa.2002.124333.View ArticlePubMedGoogle Scholar
- Fluckiger F, Ratschek M, Steiner H: Color-coded duplex sonography of dysfunctional kidney transplants. Correlation of the resistive index with the histopathologic results of biopsy. Der Radiologe. 1991, 31 (2): 51-55.PubMedGoogle Scholar
- Trillaud H, Merville P, Tran Le Linh P, Palussiere J, Potaux L, Grenier N: Color Doppler sonography in early renal transplantation follow-up: resistive index measurements versus power Doppler sonography. AJR Am J Roentgenol. 1998, 171 (6): 1611-1615. 10.2214/ajr.171.6.9843297.View ArticlePubMedGoogle Scholar
- Hidalgo LG, Campbell PM, Sis B, Einecke G, Mengel M, Chang J, Sellares J, Reeve J, Halloran PF: De novo donor-specific antibody at the time of kidney transplant biopsy associates with microvascular pathology and late graft failure. Am J Transplant: official journal of the American Society of Transplantation and the American Society of Transplant Surgeons. 2009, 9 (11): 2532-2541. 10.1111/j.1600-6143.2009.02800.x.View ArticleGoogle Scholar
- Einecke G, Sis B, Reeve J, Mengel M, Campbell PM, Hidalgo LG, Kaplan B, Halloran PF: Antibody-mediated microcirculation injury is the major cause of late kidney transplant failure. Am J Transplant: official journal of the American Society of Transplantation and the American Society of Transplant Surgeons. 2009, 9 (11): 2520-2531. 10.1111/j.1600-6143.2009.02799.x.View ArticleGoogle Scholar
- Pereira RS, Gonul II, McLaughlin K, Yilmaz S, Mahallati H: Assessment of chronic renal allograft nephropathy using contrast-enhanced MRI: a pilot study. AJR Am J Roentgenol. 2010, 194 (5): W407-W413. 10.2214/AJR.09.2912.View ArticlePubMedGoogle Scholar
- Pei XL, Xie JX: Functional MRI: evaluation of chronic kidney disease with perfusion imaging. Acad Radiol. 2009, 16 (1): 88-95. 10.1016/j.acra.2008.07.002.View ArticlePubMedGoogle Scholar
- Kim JH, Eun HW, Lee HJ, Goo DE, Choi DL: Clinical use of renal perfusion imaging by means of harmonic sonography with a microbubble contrast agent in patients after renal transplantation: preliminary study. J Ultrasound Med. 2005, 24 (6): 755-762.PubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2369/14/143/prepub
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