Stable hemoglobin in hemodialysis patients: forest for the trees – a 12-week pilot observational study
© Rottembourg et al.; licensee BioMed Central Ltd. 2013
Received: 9 September 2012
Accepted: 28 October 2013
Published: 4 November 2013
Hemoglobin (Hb) variability is a common occurrence in hemodialysis patients treated with erythropoiesis-stimulating agents. High amplitude fluctuations have been associated with greater risk of morbidity and mortality.
This prospective, single centre pilot observational study was conducted over a 3-month period in daily practice patterns, to assess per-dialysis events and inter-dialysis complications that could interfere with erythropoiesis in patients undergoing hemodialysis.
Mean Hb levels remained stable in the 78 evaluable patients, as did darbepoetin alfa (DA) doses, including in patients suffering from diabetes or cardiac affections. In total, an average of 7.7 events / patient / month occurred, but no significant relationship with Hb excursions was shown.
The observation of 7.7 events per patient per month suggests a careful monitoring of Hb and DA dosing every other week, in order to maintain Hb level within the target.
KeywordsDarbepoetin alfa Hemodialysis Hemoglobin cycling Inter-dialysis complications Per-dialysis events
Anemia is an important and common complication in patients with end-stage renal disease treated with chronic maintenance hemodialysis (HD). Its impact on cardiovascular outcomes, quality of life and even survival has been widely assessed. As a result, throughout the years, several guidelines have defined the targets in hemoglobin (Hb) levels that need to be achieved in HD patients [1–5]. These have been recently modified by the KDIGO clinical practice guidelines for anemia in chronic kidney disease (CKD) . Treatment with erythropoietin stimulating agents (ESAs) has been a major advance in the management of this problem. There is, however, a great difference between treatment and physiological erythropoietic biology, in so far as ESAs are administered episodically, generating important changes in erythropoietin – and hence – Hb levels . This phenomenon, which is commonly encountered in ESA-treated patients [8, 9], is known as Hb cycling. It can be defined as cyclical increases and decreases of measured Hb levels in individual patients . Its consequences are not fully known, however it may be accepted that Hb cycling can result in fluctuations of the oxygen carried to the tissues, and repeated episodes of relative ischemia in vital organs can lead to organ dysfunction or injury. Indeed, high amplitude swings have been found to be associated with greater risk of mortality and hospitalization .
Apart from anemia management, several factors can be accounted for causing Hb cycling: amongst others, inflammatory and infectious diseases, blood loss, hyperparathyroidism and hospitalizations [7–9]. On the other hand, little is known about dialysis-related incidents, whether they occur during or between HD sessions.
This prospective, single-center pilot observational study was conducted over a 3-month period, to assess the number and importance of per-dialysis events and inter-dialysis complications that could possibly interfere with erythropoiesis in patients undergoing hemodialysis.
Patients were treated according to routine clinical practices in the unit. As required by law, no formal approval was required by the independent ethic committee, and patients provided written informed consent prior to study entry . All patients treated in our center were included for 12 weeks in the study. They were prescribed regular HD on Integra-Hospal generators for 3.5 to 4 hours three times weekly. Vascular access was assessed by double arterio-venous fistula punctures in all patients. On start of the dialysis session, each patient received a 0.2 to 0.6 ml injection of nadroparin calcium, a low molecular weight heparin. High and medium permeability membranes were used throughout the study and were unchanged for each patient. Darbepoetin alfa (DA) injections were performed every other week (during the first dialysis session of the week) in the venous injection site before the drip chamber on the venous line (Pivipol Hospal). Any change in the dosing of DA required a follow-up of four Hb measurements, and the adaptation was made by one physician who knows the previous doses and the clinical program of the patient: if, on 4 Hb values, one was outside of the target, nothing was changed; if 2 or 3 values were outside of the target, the dosing was adapted depending the amplitude. Intravenous (IV) iron (iron sucrose [V]) was injected, if necessary, once a week during the second dialysis session at a dose ranging from 25 to 100 mg.
The follow-up conditions were those of daily practice, and the protocol ensured that no complementary visits or biological assessments would be performed outside the usual HD follow-up. Biological parameters such as Hb, calcium, phosphorus, urea, creatinine and DA dose, were assessed every other week; iron dose was recorded every week. Other parameters, and especially C-reactive protein, PTH and nutritional parameters [albumin and normalized protein catabolic rate (nPCR)] were assessed at baseline and on week 12; dialysis adequacy was assessed at the same period by Kt/V and urea reduction ratio (URR). Hb variation was defined as a ≥ 1 g/dl Hb excursion above or under baseline value and a ≥ 4 week-duration.
Upon each dialysis session, per-dialysis events, such as fistula bleeding, puncture problems, hypotension, clotting in lines or dialyzer, blood loss, and delayed coagulation, were recorded by the nurses on specific questionnaires. Physicians assessed each week inter-dialysis complications, mainly cardiovascular, digestive, infectious, hemorrhagic, and vascular access, by three means of consultation: face to face, by phone, and, when in doubt, by consulting the family.
Descriptive statistics were applied for quantitative variables, and results are reported with mean values and standard deviations; median values and ranges are also provided when appropriate, owing to dispersion of values. A uni-variate analysis was performed to assess if there is an association between events (per-dialysis and inter-dialysis) and hemoglobin excursion. The evaluable population comprised patients who attended every dialysis session during this prospective study; patients who were absent for any reason (vacation, prolonged hospitalization, graft, death) were excluded.
Demographics and characteristics at baseline
(n = 78)
Gender, n (%)
BMI (kg/m 2 )
Primary cause of renal failure, n (%)
Dialysis vintage (months)
Current history and medical treatments at baseline
Evaluable patients (n = 78)
Medical History, n (%)
Coronary artery disease
Current treatments, n (%)
ACE inhibitors and/or ARBs
VKAs, aspirin, platelet aggregation inh.
Hemoglobin and DA dose, iron dose
Hemoglobin concentrations and DA doses over time - Evaluable population (n = 78)
≥ 11 g/dl (%)
Weekly DA dose (μg/week)
Weighted weekly DA dose
Hemoglobin concentrations and DA doses over time – Cardiac and diabetic patients
Cardiac patients (n = 46)
Diabetic patients (n = 21)
11.8 (9.5- 13.2)
Weekly DA dose
Weighted weekly DA dose
Biological variables over time – Evaluable population (n = 78)
< 100 μg/l, n (%)
Transferrin saturation (%)
C-reactive protein (mg/l), n (%)
< 10 mg/l, mean(SD), (%)
4.4 (2.0), (76.4)
4.1 (1.8), (89.9)
≥ 10 mg/l, mean(SD), (%)
27.1 (19.5), (23.6)
29.8 (25.0), (10.1)
Per-dialysis events and inter-dialysis complications
Intercurrent events during the 3-month study
Total number of events
% of patients with ≥ 1 event
Mean number of events/patient/month
Clotting (lines, dialyzer)
Blood loss & delayed coagulation
Intercurrent events according to hemoglobin excursion
(N = 78)
(N = 46)
(N = 32)
Digestive tract complication
In total, an average of 7.7 events / patient / month occurred, that might interfere with erythropoiesis. There is no correlation between per-dialysis events and hemoglobin excursions (r = 0.18, p < 0.52) and inter-dialysis events and hemoglobin excursions (r = 0.21, p < 0.51).
Discussion and conclusions
Anemia, a common complication in patients with end-stage renal disease on dialysis has been shown to increase the risk of morbidity and increased mortality, and alters quality of life. Ebben and colleagues studied 152,846 Medicare hemodialysis patients and defined six patients groups on the basis of hemoglobin level fluctuation . The consistently low group (Hb < 11 g/dl) had the highest percentage of hospitalizations and the highest number of co-morbid conditions. On the other hand, the CHOIR trial, in patients not on dialysis, showed that targeting a hemoglobin level of 13.5 g/dl was associated with a significantly increased risk of a composite endpoint of death, myocardial infarction, hospitalization for congestive heart failure, and stroke, without any significant improvement in quality of life . A same figure was observed on hemodialysis in the Normal Hematocrit Study . It appears that both low and high Hb levels are associated with increased mortality risk, in a U-shaped or inverse J-curve relationship . Somewhat conflicting results have been published, however: in a 2-year duration study of 5,037 European HD patients, Eckard and colleagues determined Hb variability, and concluded that, although it is frequently seen in these patients, it does not independently predict mortality .
The findings of our study support the hypothesis that patient-related factors such as per-dialysis events, inter-dialysis events and co-morbid conditions could influence the degree of Hb variability in patients undergoing HD, if the monitoring of Hb and the dosing of DA are done with an interval exceeding two weeks. A total of 1516 per-dialysis events such as fistula bleeding, puncture problems, hypotension, clotting in the lines or dialyzer or delayed coagulation resulting in blood loss were observed along with 279 inter-dialysis events such as cardiovascular, infectious hemorrhagic and vascular access complications that did not lead to hospitalizations, or for no more than two days. This resulted in a mean 7.7 events per month per patient potentially interfering with erythropoiesis.
Defining an optimal level for Hb patients with CKD is therefore difficult. This may explain why guidelines have evolved during the past decade, recommending a target of 10 to12 g/dl for the latest KDOQI guidelines [16, 17], which is narrower for ERBP (11–12 g/dl) , and much higher than the new KDIGO (9.5-11.5 g/dl) .
A small number of HD patients, however, have normal Hb levels and have no need for ESAs. A population of 45 non anemic HD patients was compared to a control group of 205 HD patients on ESA therapy . Absence of anemia was more frequent in men and younger patients with long-term renal replacement therapy, in patients with HCV + liver disease and adult polycystic kidney disease, associated with increased endogenous erythropoietin production and renal and hepatic cysts. In our study, 7 of the 78 evaluable patients did not receive any ESA therapy, 3 of them had a polycystic kidney disease, 3 were diabetics and one had a nephrosclerosis.
More than a set target level, however, fluctuations in Hb levels have been shown to be associated with co-morbid situations. This frequent phenomenon, known as hemoglobin cycling, can be defined as fluctuations over time in measured Hb levels, increasing above or decreasing below the target and reversing direction . Apparently stable mean Hb levels in an overall study population can hide the occurrence of intra-individual variability in many patients. In a retrospective data analysis of 281 HD patients receiving ESAs, an occurrence of Hb cycling in 90% of patients has been reported . Lacson and colleagues reported important fluctuations over time, with only 5% of ESRD patients in the target range during a 6-month period observation . In another retrospective study, undertaken in the Netherlands, none of the 97 ESA-treated patients had Hb levels stable within the target range over a one-year period . In our present study, we report stable levels of hemoglobin as a whole in the evaluable population, as well as in the cardiac and diabetic subgroups, during the whole study period. However 54% of the patients needed at least one change in darbepoetin alfa dose, to compensate Hb fluctuations outside the target level. In our unit, over the last few years, the median number of darbepoetin alfa dose changes per year is about three .
Numerous causes have been identified that can result in Hb cycling. Some are patient-related, such as age (older patients show smaller variability) , co-morbid conditions, e.g. inflammatory states or infections , levels of parathyroid hormone, and concurrent hematologic disorders. In HD patients, red blood cells have a short circulating half-life, due to fluctuations in erythropoietin levels, and this is a component of variability in Hb levels .
Other causes of Hb variability are drug-related: ESAs have been a major advance in anemia management, but also contribute to Hb cycling, and compounds pharmacokinetics, dosing and frequency of administration generate substantial changes in erythropoiesis [25–27]. For Portoles, long-acting ESA, darbepoetin alfa, achieved better Hb stability than short-acting ESA . Iron homeostasis also plays an important role in anemia management . If the etiology of Hb variability is not easily discerned, erythropoietic hyporesponsiveness must be envisaged and evaluated, and all efforts brought to reaching stability [30, 31].
Hb testing procedures, practice patterns and reimbursement policies can also contribute to Hb fluctuations. Publications have mentioned center-effects: a retrospective observational study conducted on the US Renal Data System (USRDS) in 2000 and 2001 identified a wide center variation in hematocrit, with a mean difference of 3.06 between the poorest versus the best performing units .
All these factors can thus contribute to intra-patient variability. Amongst them, intercurrent events, such as inter-dialysis complications and per-dialysis events have been little studied. De Francisco and colleagues carried out a post-hoc analysis of Hb concentrations on the pooled records of 5,592 HD patients included in Phase 3b trials on the efficacy and safety of darbepoetin alfa for the management of anemia . The intra-patient variability was significantly greater in the presence of infection or inflammation, blood transfusion, or hospitalization including for cardiovascular causes. Little is known about the per-dialysis events which are the most frequent events observed in the HD population: compared to patients treated by peritoneal dialysis, the Hb level is less stable in patients treated by HD  and the requirement of ESAs doses much higher in the HD population [34, 35]. Moreover inter dialysis hypotension episodes, which are one of the most frequent events, are known to disturb hemoglobin stability , and patients suffering of frequent intradialytic hypotensive episodes present a hemoglobin level lower than that of the stable patients .
It has been advised that strategies to minimize Hb cycling should focus on individualizing targets for the patients. Targets defined by the guidelines are convenient for most patients. However, authors have recommended that the therapeutic decision should center on what Hb is most appropriate at a “safe ESA dose”, even if it means a target of 12 to 13 g/dl .
In our experience, in order to achieve and stabilize Hb levels within a safe range, we use moderate DA doses and address the ongoing iron needs of the patients, now injected on the same Q2W basis than DA . Monitoring both Hb and DA dosing every other week appears to be the most sensible way to achieve this goal. However, our study is a pilot study, and needs to be confirmed by wider multicenter trials.
The authors would like to thank Marie-Danielle Bernard, MD (Anticipsanté, Marly le Roi, France), who assisted in writing the manuscript. Her assistance was funded by Amgen S.A.S. (France).
- National Kidney Foundation: NKF-DOQI clinical practice guidelines for the treatment of anemia of chronic renal failure. Am J Kidney Dis. 1997, 30 (Suppl 3): S192-S230.Google Scholar
- National Kidney Foundation: NKF-K/DOQI clinical practice guidelines for anemia of chronic kidney diseases: update 2000. Am J Kidney Dis. 2001, 37 (Suppl 1): S182-S238.Google Scholar
- Locatelli F, Aljama P, Barany P, Canaud B, Carrera F, Eckardt KU, Hörl WH, Macdougal IC, Macleod A, Wiecek A, Cameron S: Revised European best practice guidelines for the management of anaemia in patients with chronic renal failure. Nephrol Dial Transplant. 2004, 19 (Suppl 2): ii1-ii47.PubMedGoogle Scholar
- KDOQI: Clinical practice guidelines and clinical practice recommendations for anemia in chronic kidney disease. Am J Kidney Dis. 2006, 47: S11-S147.View ArticleGoogle Scholar
- KDOQI: Clinical practice guidelines and clinical practice recommendations for anemia in chronic kidney disease – 2007 update of haemoglobin target. Am J Kidney Dis. 2007, 50: 471-530.View ArticleGoogle Scholar
- KDIGO: Clinical practice guidelines for anemia in chronic kidney disease. Kidney Int Suppl. 2012, 2: 280-335.View ArticleGoogle Scholar
- Fishbane S, Berns JS: Evidence and implications of haemoglobin cycling in anaemia management. Nephrol Dial Transplant. 2007, 22: 2129-2132. 10.1093/ndt/gfm384.View ArticlePubMedGoogle Scholar
- Berns JS, Elzein H, Lynn RI, Fishbane S, Meisels IS, Deoreo PB: Hemoglobin variability in epoetin-treated hemodialysis patients. Kidney Int. 2003, 64: 1514-1521. 10.1046/j.1523-1755.2003.00229.x.View ArticlePubMedGoogle Scholar
- Fishbane S, Berns JS: Hemoglobin cycling in hemodialysis patients treated with recombinant human erythropoietin. Kidney Int. 2005, 68: 1337-1343. 10.1111/j.1523-1755.2005.00532.x.View ArticlePubMedGoogle Scholar
- Ebben JP, Gilbertson DT, Foley RN, Collins AJ: Hemoglobin level variability: associations with comorbidity, intercurrent events, and hospitalisations. Clin J Am Soc Nephrol. 2006, 1: 1205-1210. 10.2215/CJN.01110306.View ArticlePubMedGoogle Scholar
- Journal Officiel de la République Française. Décret N° 2006–477 du 26/04/2006, paru au JO du 27/04/2006. 2006, http://www.legifrance.gouv.fr,
- Singh AK, Szczech L, Tang KL, for the CHOIR investigators: Correction of anemia with epoetin alfa in chronic kidney disease. N Engl J Med. 2006, 355: 2085-2098. 10.1056/NEJMoa065485.View ArticlePubMedGoogle Scholar
- Besarab A, Bolton WK, Browne JK, Egrie JC, Nissenson AR, Okamoto DM, Schwab SJ, Goodkin DA: The effects of normal as compared with low hematocrit values in patients with cardiac disease who are receiving hemodialysis and epoetin. N Engl J Med. 1998, 339: 584-590. 10.1056/NEJM199808273390903.View ArticlePubMedGoogle Scholar
- Kalantar-Zadeh K, Aronoff GR: Hemoglobin variability in anemia of chronic kidney disease. J Am Soc Nephrol. 2009, 119: 479-487.View ArticleGoogle Scholar
- Eckardt KU, Kim J, Kronenberg F, Aljama P, Anker SD, Canaud B, Molemans B, Stenvinkel P, Schernthaner G, Ireland E, Fouqueray B, Macdougall IC: Hemoglobin variability does not predict mortality in European hemodialysis patients. J Am Soc Nephrol. 2010, 21: 1765-1775. 10.1681/ASN.2009101017.View ArticlePubMedPubMed CentralGoogle Scholar
- Steinbrock R: Haemoglobin concentrations in chronic kidney disease. Lancet. 2006, 368: 2191-2193. 10.1016/S0140-6736(06)69707-9.View ArticleGoogle Scholar
- Levin A: Understanding recent haemoglobin trials in CKD: methods and lessons from CREATE and CHOIR. Nephrol Dial Transplant. 2007, 22: 309-312.View ArticlePubMedGoogle Scholar
- Locatelli F, Aljama P, Canaud B, Covic A, De Francisco A, Macdougall IC, Wiecek A, Vanholder R: Target haemoglobin to aim for with erythropoiesis –stimulating agents: a position statement by ERBP following publication of the Trial to Reduce Cardiovascular Events with Aranesp® Therapy (TREAT) Study. Nephrol Dial Transplant. 2010, 25: 2846-2850. 10.1093/ndt/gfq336.View ArticlePubMedGoogle Scholar
- Verdalles U, Abad S, Vega A, Ruiz Caro C, Ampuero J, Jofre R, Lopez-Gomez JM: Factors related to the absence of anemia in hemodialysis patients. Blood Purif. 2011, 32: 69-74. 10.1159/000323095.View ArticlePubMedGoogle Scholar
- Lacson E, Ofsthun N, Lazarus JM: Effect of variability in anemia management on haemoglobin outcomes in ESRD. Am J Kidney Dis. 2003, 41: 111-124. 10.1053/ajkd.2003.50030.View ArticlePubMedGoogle Scholar
- Van der Putten K, van der Baan F, Schellekens H, Gaillard C: Hemoglobin variability in patients with chronic kidney disease in the Netherlands. Int J Artif Organs. 2009, 32: 787-793.PubMedGoogle Scholar
- Rottembourg JB, Dansaert A: Strategy feasibility of darbepoetin alfa administration every other week: 2005–2007 experience in a dialysis unit. Nephrol Ther. 2011, 7: 549-557. 10.1016/j.nephro.2011.03.011.View ArticlePubMedGoogle Scholar
- De Francisco AL, Macdougall IC, Carrera F, Braun J, Bárány P, Bridges I, Wheeler T, Tran D, Dietrich A: Intercurrent events and comorbid conditions influence hemoglobin level variability in dialysis patients. Clin Nephrol. 2009, 71: 397-404. 10.5414/CNP71397.View ArticleGoogle Scholar
- Handelman GJ, Levin NW: Red cell survival: relevance and mechanism involved. J Ren Nutr. 2010, 20 (5 Suppl): S84-88.View ArticlePubMedGoogle Scholar
- Walker R, Pussell BA: Fluctuations in haemoglobin levels in haemodialysis, pre-dialysis and peritoneal dialysis patients receiving epoetin alpha or darbepoetin alpha. Nephrol. 2009, 14: 689-695. 10.1111/j.1440-1797.2009.01166.x.View ArticleGoogle Scholar
- Unger EF, Thompson AM, Blank MJ, Temple R: Erythropoiesis-stimulating agents–time for a reevaluation. N Engl J Med. 2010, 362: 189-192. 10.1056/NEJMp0912328.View ArticlePubMedGoogle Scholar
- Roche A, Macdougall IC, Walker RG: Haemoglobin fluctuations in patients on haemodialysis treated with ESAs: clinical observations from two centers. Curr Med Res Opin. 2009, 25: 2971-2976. 10.1185/03007990903350029.View ArticlePubMedGoogle Scholar
- Portoles JM, de Francisco ALM, Gorriz JL, Martinez-Castelao A, Lopez-Gomez JM, Arias M, de la Cruz JJ, Cases A, Fernandes E, Aljama P: Maintenance of target hemoglobin level in stable hemodialysis patients constitutes a theoretical task : a historical prospective study. Kidney Int. 2008, 74 (Suppl 111): S82-S87.View ArticleGoogle Scholar
- Kovesdy CP: Iron and clinical outcomes in dialysis and non-dialysis-dependent chronic kidney disease patients. Adv Chronic Kidney Dis. 2009, 16: 109-116. 10.1053/j.ackd.2008.12.006.View ArticlePubMedGoogle Scholar
- Yee J, Zasuwa G, Frinak S, Besarab A: Hemoglobin variability and hyporesponsiveness: much ado about something or nothing?. Adv Chronic Kidney Dis. 2009, 16: 83-93. 10.1053/j.ackd.2008.12.003.View ArticlePubMedGoogle Scholar
- Kainz A, Mayer B, Kramar R, Oberbauer R: Association of ESA hypo-responsiveness and haemoglobin variability with mortality in haemodialysis patients. Nephrol Dial Transplant. 2010, 25: 3701-3706. 10.1093/ndt/gfq287.View ArticlePubMedPubMed CentralGoogle Scholar
- Fink JC, Hsu VD, Zhan M, Walker LD, Mullins CD, Jones-Burton C, Langenberg P, Seliger SL: Center effects in anemia management of dialysis patients. J Am Soc Nephrol. 2007, 18: 646-653. 10.1681/ASN.2006050433.View ArticlePubMedGoogle Scholar
- Rao R, Ansell D, Gilg JA, Davies SJ, Lamb EJ, Tomson CR: Effect of change in renal replacement therapy modality on laboratory variables: a cohort study from the UK Renal Registry. Nephrol Dial Transplant. 2009, 24: 2877-2882. 10.1093/ndt/gfp163.View ArticlePubMedGoogle Scholar
- United States Renal Data System. The concise. 2010, http://www.usrds.org/archive.aspx, annual data report,
- Feriani M, De Meester JMJ, McMahon LP, Rottembourg JB, Bridges I, Farouk M, Pronai W: Extended dosing of darbepoetin alfa in peritoneal dialysis patients. BMC Nephrol. 2011, 2: 13-21.View ArticleGoogle Scholar
- Rouby JJ, Rottembourg J, Durande JP: Hemodynamic changes induced by regular hemodialysis and sequential ultrafiltration hemodialysis: a comparative study. Kidney Int. 1980, 17: 801-810. 10.1038/ki.1980.92.View ArticlePubMedGoogle Scholar
- Schroeder KL, Sallustio JE, Ross EA: Continuous haematocrit monitoring during intradialytic hypotension: precipitous decline in plasma refill rates. Nephrol Dial Transplant. 2004, 19: 652-656. 10.1093/ndt/gfg590.View ArticlePubMedGoogle Scholar
- Besarab A, Frinak S, Yee J: What is so bad about a hemoglobin level of 12 to 13 g/dL for chronic kidney disease patients anyway?. Adv Chronic Kidney Dis. 2009, 16: 131-142. 10.1053/j.ackd.2008.12.007.View ArticlePubMedGoogle Scholar
- Rottembourg J, Guerin A, Diaconita M: Anemia day: simultaneous, once every two weeks (Q2W) administrations of darbepoetin alfa (DA) and iron (V), improve anemia management in hemodialysis (HD) patients. 2011, Philadelphia: Abstract and Poster presented at the Annual Meeting of the American Society of Nephrology, FR-PO1596Google Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2369/14/243/prepub
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