Impaired P2Y12 inhibition by clopidogrel in kidney transplant recipients: results from a cohort study
- Clotilde Muller†1, 3, 4,
- Nathan Messas†2,
- Peggy Perrin1, 4,
- Jerome Olagne1,
- Gabriela Gautier-Vargas1, 3,
- Noelle Cognard1, 3,
- Sophie Caillard1, 3, 4,
- Bruno Moulin1, 3, 4 and
- Olivier Morel2, 3, 5Email author
© The Author(s). 2016
Received: 16 September 2015
Accepted: 1 June 2016
Published: 9 June 2016
Cardiovascular complications represent a major cause of morbidity and mortality for patients who received kidney transplantation (KT). However, the impact of KT and chronic immunosuppression on platelet response to clopidogrel in patients undergoing coronary or peripheral revascularization procedures remains unclear. This cohort study compares platelet responsiveness to clopidogrel as assessed byvasodilator-stimulated phosphoprotein (VASP) phosphorylation.
The study population was divided between chronic kidney disease (CKD) patients who underwent KT (n = 36) and non-transplanted CKD patients (control group, n = 126). Patients were on maintenance antiplatelet therapy with clopidogrel 75 mg daily for at least 8 days. The mean platelet reactivity index (PRI) VASP values and the prevalence of high on-treatment platelet reactivity (HPR, defined as PRI VASP ≥61 %) were compared.
The mean PRI VASP value was significantly higher in the transplant group (60.1 ± 3 vs 51.2 ± 1.6 %; p=0.014). HPR was significantly more common in the transplant group on clopidogrel maintenance therapy (58 vs. 31 %; p = 0.011). KT was the only independent predictor of HPR (odds ratio: 2.6; 95 % confidence interval: 1.03–6.27, p = 0.03). The effect of treatment with calcineurin inhibitors on clopidogrel response could not be analyzed separately from the kidney transplant status.
KT is associated with an increased prevalence of HPR. Our results suggest that plateletfunction tests may be clinically useful for the management of this specific population.
KeywordsCardiovascular disease Kidney transplantation Platelet aggregation
Chronic kidney disease (CKD) portends an increased risk of cardiovascular morbidity and mortality [1–7]. Moreover, a decreased glomerular filtration rate (GFR) has been associated with unfavourable clinical outcomes in patients undergoing percutaneous coronary intervention (PCI), possibly because of endothelial dysfunction and platelet activation [8–10]. Dual anti-platelet therapy with aspirin and P2Y12 receptor antagonists (including clopidogrel) is currently considered as a key strategy to reduce platelet reactivity and to prevent thrombotic complications following PCI [11, 12]. However, the clinical utility of clopidogrel may be hampered by the high inter-individual variability of inhibition of P2Y12-dependent platelet function. Accordingly, an elevated on-clopidogrel platelet reactivity (HPR) to adenosine diphosphate is a well-known risk factor for adverse events (especially stent thrombosis) following PCI [13–18]. In CKD patients, a significant and detrimental relationship between the severity of renal impairment and the extent of P2Y12 inhibition has been reported [19, 20]. Cardiovascular complications represent a major cause of morbidity and mortality for CKD patients undergoing kidney transplantation (KT) . Notably, a previous investigation reported an association of clopidogrel use with a higher mortality risk, although caution should be exercised as it was an observational study with some data missing . An increased platelet activation caused by calcineurin inhibitors has been advocated to explain the increased atherothrombotic risk observed in renal allograft recipients [23, 24]. However, the impact of KT and chronic immunosuppression on platelet response to clopidogrel in CKD patients undergoing coronary or peripheral revascularization procedures remains unclear. We therefore designed the current cohort study in order to compare platelet responsiveness to clopidogrel [as assessed by vasodilator-stimulated phosphoprotein (VASP) phosphorylation] in KT recipients and non-transplanted CKD patients.
Between November 2011 and January 2013, a total of 162 CKD patients undergoing coronary or peripheral stenting in our institution were retrospectively examined. Patients on maintenance antiplatelet therapy with clopidogrel 75 mg daily for at least 8 days were deemed eligible. Subjects with contraindications to antiplatelet therapy, platelet count <100,000/ml, or history of bleeding were excluded. Clinical interviews to assess adherence to antiplatelet therapy were systematically performed before inclusion. The study patients were divided into two groups, i.e., CKD patients who underwent KT (transplant group, n = 36) and non-transplanted CKD patients (control group, n = 126). Patients included in the transplant group were transplanted from March 1991 to December 2012. The reasons for transplantation were as follows: glomerulonephritis (n = 14), diabetic nephropathy (n = 9), genetic disease (n = 5), unclassified (n = 3), anomalies of the kidney and urinary tract (n = 2), and chronic interstitial nephritis (n = 2), and nephroangiosclerosis (n = 1). Patients who underwent KT received immunosuppressive drugs; specifically, calcineurin inhibitors and mycophenolate mofetil were given to 89 and 86 % of the study patients, respectively. Transplanted patients were on clopidogrel for more than 8 days (they were all on chronic therapy, introduced before or after transplantation) as in the control group. There was no patient follow up.
Assessment of renal function
Baseline serum creatinine levels were determined in all participants at inclusion using isotope dilution mass spectrometry (IDMS), concomitantly with the platelet function tests. All samples were collected in the morning. The estimated glomerular filtration rate (eGFR) was calculated using the Modification of Diet in Renal Disease (MDRD) formula, as follows: eGFR = 175 × (creatinine (μmol/L) × 0.0113)-1.154 × age- 0.203 (×0.74 for women). CKD was defined as an eGFR <60 mL/min/1.73 m2. In the transplant group, results were based on the post-transplant renal function.
Blood sampling and platelet function tests
Whole blood samples were collected by venipuncture into Vacutainer tubes (BD Vacutainer, Becton Dickinson, Sparks, MD, USA) containing 0.129 M sodium citrate and immediately sent to the hemostasis laboratory (EFS-Alsace) for the assessment of VASP phosphorylation status. Platelet VASP phosphorylation was determined with a standardized flow cytometric assay (Biocytex Platelet VASP kit, Marseille, France) as previously described . Levels of VASP phosphorylation were expressed as the platelet reactivity index (PRI) calculated from the median fluorescence intensity of the samples incubated with prostaglandin E1 alone or prostaglandin E1 and adenosine diphosphate according to the following formula: PRI = [(median fluorescence intensity prostaglandin E1 – median fluorescence intensity prostaglandin E1 + adenosine diphosphate)/median fluorescence intensity prostaglandin E1]/100. The main advantages of the VASP assay include its selectivity for the P2Y12 pathway, its insensitivity towards glycoprotein IIb/IIIa inhibitors, the stability of the results for >24 h after blood sampling, and the interpretability of single measurements. We have previously shown by receiver-operating characteristic (ROC) curve analysis that 61 % is the optimal cut-off value for PRI in the prediction of adverse cardiovascular events in patients undergoing PCI (including those with CKD) . Consequently, patients with a PRI VASP ≥61 % were considered to have a high on-clopidogrel platelet reactivity to clopidogrel (i.e., low responders).
The following three pharmacodynamic endpoints were considered for this study: 1) analysis of platelet response to clopidogrel through the comparison of mean PRI VASP values in KT recipients and non-transplanted CKD patients; 2) prevalence of HPR (using a cut-off value for PRI VASP ≥61 %) in KT recipients and non-transplanted CKD patients; and 3) identification of the main predictors of HPR in the study participants.
Continuous variables are expressed as means ± standard deviations (SD) and compared with the Student’s t-test (Gaussian data) or the Mann–Whitney U test (skewed variables). Categorical variables are presented as counts and percentages and compared with χ 2 test. One-way analysis of variance was used for intergroup comparisons and calculation of trend tests. Univariate and multivariate logistic regression analyses were performed to identify the main predictors of HPR. Variables with a p value <0.20 on univariate analyses were entered in blocks into the multivariate model. Results are presented as odds ratios (ORs) with their 95 % confidence intervals (CIs). All computations were performed using the SPSS statistical software, version 17.0 (SPSS Inc., Chicago, IL, USA). Two-tailed p values <0.05 were considered statistically significant. When multiple comparison tests were performed, the statistical significance threshold was corrected using the Bonferroni’s method. Missing data were not replaced (deletion).
Baseline characteristics of the study patients
Baseline characteristics of the study patients
Transplant group (n = 36)
Control group (n = 126)
58.3 ± 1.6
72.6 ± 1.1
Male sex, n (%)
26 (72 %)
79 (63 %)
Cardiovascular risk factors
Diabetes mellitus, n (%)
14 (39 %)
73 (58 %)
Hypertension, n (%)
33 (92 %)
108 (86 %)
Current smoking, n (%)
14 (39 %)
60 (48 %)
Hypercholesterolemia, n (%)
30 (83 %)
104 (83 %)
Body mass index (kg/m2)
25.2 ± 0.8
27.2 ± 0.5
eGFR (MDRD formula) (mL/min/1.73 m2)
47.9 ± 3.7
39.5 ± 2.9
Anemia (Hb <12.5 g/dL), n (%)
17 (47 %)
68 (54 %)
7.6 ± 1.5
25.4 ± 4.1
Pharmacodynamic results: biological response to clopidogrel
Univariate logistic regression analysis for the prediction of high on-clopidogrel platelet reactivity (PRI VASP ≥61 %)
CI (95 %)
Body mass index (kg/m2)
eGFR (mL/min/1.73 m2)
Calcineurin inhibitors use
Proton pump inhibitor use
Predictors of high on-treatment platelet reactivity
Multivariate logistic regression analysis for the prediction of high on clopidogrel platelet reactivity (PRI VASP ≥61 %)
CI (95 %)
Body mass index (kg/m2)
eGFR (mL/min/1.73 m2)
This study highlighted three principal findings. First, we identified a high prevalence of low response to clopidogrel in CKD patients. Second, we observed an increased prevalence of high on-clopidogrel platelet reactivity in CKD patients who underwent KT. Finally, we identified KT as the only independent predictor of HPR in our study patients.
Platelet response to clopidogrel in CKD patients: role of kidney transplantation
CKD patients have an increased risk of cardiovascular complications and represent a sizeable proportion of the population undergoing percutaneous coronary or peripheral revascularization procedures [1–7]. Several reports and registry studies focusing either on acute coronary syndromes or elective PCI have shown that CKD patients have higher rates of ischemic and post-procedural adverse events [27–29]. The mechanisms through which CKD may exert an unfavorable impact on clinical outcomes following coronary interventions are probably multifactorial (e.g., endothelial dysfunction, platelet activation, chronic inflammation, and presence of comorbidities). The exact role played by the impaired response to P2Y12 blockade in the pathogenesis of atherothrombotic complications among CKD patients remains unclear. Recent studies demonstrated that a decreased GFR is significantly associated with a poor response to thienopyridine therapy [26, 30]. We and others have also reported a significant interaction between CKD and high on-clopidogrel platelet reactivity (defined as a PRI VASP ≥61 %), with the latter parameter being a strong independent predictor of cardiac death [20, 31].
To the best of our knowledge, the magnitude of platelet inhibition by clopidogrel in CKD patients undergoing KT has not been previously investigated. The current study extends previous reports demonstrating a noxious interaction between KT and impaired platelet inhibition by clopidogrel. Herein, we have shown that a history of KT was an independent predictor factor of high platelet reactivity under clopidogrel treatment even after allowance for traditional risk factors for HPR (e.g., age, diabetes, or BMI). Notably, diabetes mellitus – a major factor for impaired platelet inhibition by clopidogrel – was significantly more common in non-transplanted CKD patients. Another important finding in the current study is the identification of an increased BMI as a risk factor for HPR. We and others have previously demonstrated the relevance of obesity as an independent predictor of HPR under clopidogrel treatment, regardless of the presence of diabetes [32, 33]. Because clopidogrel is metabolized in a lipophilic compound, we cannot exclude its diffusion within the adipose tissue, ultimately resulting in reduced circulating concentrations among overweight patients.
Impact of immunosuppressive therapy on high on-treatment platelet reactivity in renal transplant recipients
Cardiovascular disorders continue to represent the leading cause of morbidity and mortality in CKD patients undergoing kidney transplantation [34, 35]. Observational registries have reported a 46-fold increase in cardiovascular mortality in this patient group compared with the general population [36, 37]. Besides conventional cardiovascular risk factors, chronic immunosuppression may play a significant role in increasing atherothrombotic risk both by promoting endothelial dysfunction and a chronic prothrombotic state. Accordingly, a recent study conducted on 62 KT patients suggested that calcineurin inhibitors may exert detrimental effects on both platelet and endothelial function. Specifically, chronic cyclosporine A and, in a lesser way tacrolimus administration may result in platelet activation and endothelial dysfunction, as there is an increase in parameters of platelet degranulation (CD62P), activation (PAC-1), platelet leukocyte aggregate formation (CD41 et CD11b) and inflammatory response (soluble CD40 ligand) [23, 24]. Similarly, treatment with mycophenolate mofetil has been shown to increase plasma concentrations of platelet activation markers (e.g., CD62) . However, the exact pathophysiological mechanisms linking immunosuppression and platelet response to P2Y12 blockade remain unclear. The presence of interactions between immunosuppressive and antiplatelet medications cannot be excluded. Clopidogrel is a prodrug that requires hepatic conversion to exert its antiplatelet effects. Most of the absorbed clopidogrel (85 %) is hydrolyzed by an esterase to an inactive metabolite, whereas the remaining 15 % is rapidly metabolized by hepatic cytochrome P450 via a two-step process . Because calcineurin inhibitors are similarly metabolized by cytochrome P450, it is possible that an increased hepatic metabolism may ultimately reduce the fraction of the active metabolite. Another possible explanation may involve transporter-based mechanisms (e.g., P-glycoprotein; P-gp). P-gp is expressed by enterocytes and mediates the ATP-dependent active transports of various drugs, including the intestinal efflux of clopidogrel. Notably, cyclosporine A may act as a potent inhibitor of P-gp . We therefore speculate that the reduced response to clopidogrel observed in KT recipients may result from impaired drug absorption, altered metabolization, or both, ultimately resulting in lower levels of circulating active metabolites.
Because of sample size limitations, our study was not designed to assess the impact of HPR on hard clinical endpoints. We nonetheless believe that the high prevalence (up to 58 %) of impaired platelet inhibition to the 75 mg/day clopidogrel maintenance dose in KT recipients may be clinically relevant. Because HPR is common in renal transplant recipients, platelet function tests should be carefully considered for patients undergoing PCI, especially in presence of a positive history of atherothrombotic events despite dual antiplatelet therapy. Newer agents can be also used in patients who show significantly increased residual platelet aggregation following standard treatment. In this scenario, the potential advantages of the non-thienopyridine P2Y12 receptor antagonist ticagrelor (that does not require biotransformation) in KT recipients deserve further scrutiny, especially in presence of acute coronary syndromes. Notably, ticagrelor has been found to reduce mortality and the incidence of ischemic vascular events in a CKD population with an acceptable bleeding risk .
Some caveats of our study merit comment. Our observational registry has a single-center nature. Moreover, all laboratory results were from single-point measurements (a common limitation of studies assessing response to P2Y12 blockade). In the absence of analytical data, we cannot firmly establish the presence of reduced levels of clopidogrel active metabolite in CKD patients undergoing KT. The timing of VASP assessment was not uniform in the study patients. Moreover, we did not specifically compare the VASP assay with other platelet function tests (e.g., VerifyNow, platelet agreggometry). Concerning patients with high residual platelet reactivity, we did not specifically investigate the pharmacodynamic effects of higher clopidogrel dosages or novel P2Y12 inhibitors (e.g., prasugrel, ticagrelor). Because of sample size limitations, the current study was not adequately powered to draw firm conclusions on the potential impact of HPR on clinically hard endpoints. Because the great majority of KT patients were being treated with calcineurin inhibitors, we believe that the impact of therapy with calcineurin inhibitors on clopidogrel response could not be analyzed separately from the kidney transplant status.
As a conclusion, KT is associated with an increased prevalence of high on-clopidogrel platelet reactivity. Our results suggest that platelet function tests may be clinically useful for the management of this specific population. However, the clinical impact and the precise mechanisms of this interaction remain to be established. Future studies should assess whether a tailored antiplatelet therapy based on PRI VASP monitoring may significantly improve clinical outcomes in CKD patients undergoing KT.
BMI, body mass index; CI, confidence interval; CKD, chronic kidney disease; GFR, glomerular filtration rate; HPR, high on-clopidogrel platelet reactivity; IDMS, isotope dilution mass spectrometry; KT, kidney transplantation; MDRD, Modification of diet in renal disease; OR, odds ratio; PCI, percutaneous coronary intervention; PRI, platelet reactivity index; SD, standard deviation; VASP, vasodilator-stimulated phosphoprotein
Availability of data and materials
The data supporting our findings will be shared upon request.
CM, SC, BM and OM have participated to the conception or design, or analysis and interpretation of data, or both. CM, NM, OM contributed to drafting the article or revising it. CM, NM, OM, SC, JO, GG-V, NC, and PP have provided intellectual content of critical importance to the work described. All authors gave their final approval of the version to be published.
The authors declare that they have no competing interests.
Ethics approval and consent to participate
The study protocol was approved by the local Institutional Review Board (Comité de Protections des Personnes EST IV, Strasbourg, France). The study was performed in accordance with the Declaration of Istanbul 2008 and all participants provided their informed consent prior to their inclusion in the study.
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- Weiner DE, Tighiouart H, Amin MG, Stark PC, MacLeod B, Griffith JL, et al. Chronic kidney disease as a risk factor for cardiovascular disease and all-cause mortality: a pooled analysis of community-based studies. J Am Soc Nephrol. 2004;15(5):1307–15.View ArticlePubMedGoogle Scholar
- Manjunath G, Tighiouart H, Ibrahim H, MacLeod B, Salem DN, Griffith JL, et al. Level of kidney function as a risk factor for atherosclerotic cardiovascular outcomes in the community. JAC. 2003;41(1):47–55.Google Scholar
- Dumaine R, Collet J-P, Tanguy M-L, Mansencal N, Dubois-Randé J-L, Henry P, et al. Prognostic significance of renal insufficiency in patients presenting with acute coronary syndrome (the Prospective Multicenter SYCOMORE study). AJC. 2004;94(12):1543–7.View ArticleGoogle Scholar
- Gibson CM, Dumaine RL, Gelfand EV, Murphy SA, Morrow DA, Wiviott SD, et al. Association of glomerular filtration rate on presentation with subsequent mortality in non-ST-segment elevation acute coronary syndrome; observations in 13,307 patients in five TIMI trials. Eur Heart J. 2004;25(22):1998–2005.View ArticlePubMedGoogle Scholar
- Santopinto JJ, Fox KAA, Goldberg RJ, Budaj A, Piñero G, Avezum A, et al. Creatinine clearance and adverse hospital outcomes in patients with acute coronary syndromes: findings from the global registry of acute coronary events (GRACE). Heart. 2003;89(9):1003–8.View ArticlePubMedPubMed CentralGoogle Scholar
- Go AS, Chertow GM, Fan D, McCulloch CE, Hsu C-Y. Chronic kidney disease and the risks of death, cardiovascular events, and hospitalization. N Engl J Med. 2004;351(13):1296–305.View ArticlePubMedGoogle Scholar
- Surana SP, Riella LV, Keithi-Reddy SR, Charytan DM, Singh AK. Acute coronary syndrome in ESRD patients. Kidney Int. 2008;75(5):558–62.View ArticlePubMedGoogle Scholar
- Best PJM, Lennon R, Ting HH, Bell MR, Rihal CS, Holmes DR, et al. The impact of renal insufficiency on clinical outcomes in patients undergoing percutaneous coronary interventions. JAC. 2002;39(7):1113–9.Google Scholar
- Naidu SS, Selzer F, Jacobs A, Faxon D, Marks DS, Johnston J, et al. Renal insufficiency is an independent predictor of mortality after percutaneous coronary intervention. AJC. 2003;92(10):1160–4.View ArticleGoogle Scholar
- Angiolillo DJ, Fernandez-Ortiz A, Bernardo E, Alfonso F, Macaya C, Bass TA, et al. Variability in individual responsiveness to clopidogrel: clinical implications, management, and future perspectives. J Am Coll Cardiol. 2007;49(14):1505–16.View ArticlePubMedGoogle Scholar
- Kolh P, Windecker S. ESC/EACTS myocardial revascularization guidelines 2014. Eur Heart J. 2014;35(46):3235–6.Google Scholar
- Task Force on the management of ST-segment elevation acute myocardial infarction of the European Society of Cardiology (ESC), Steg PG, James SK, Atar D, Badano LP, Blömstrom-Lundqvist C, et al. ESCGuidelines for the management of acute myocardial infarction in patients presenting with ST-segmentelevation. Eur Heart J. 2012;33(20):2569–619.Google Scholar
- Cuisset T, Frere C, Quilici J, Barbou F, Morange PE, Hovasse T, et al. High post-treatment platelet reactivity identified low-responders to dual antiplatelet therapy at increased risk of recurrent cardiovascular events after stenting for acute coronary syndrome. J Thromb Haemost. 2006;4(3):542–9.View ArticlePubMedGoogle Scholar
- Geisler T, Langer H, Wydymus M, Göhring K, Zürn C, Bigalke B, et al. Low response to clopidogrel is associated with cardiovascular outcome after coronary stent implantation. Eur Heart J. 2006;27(20):2420–5.View ArticlePubMedGoogle Scholar
- Hochholzer W, Trenk D, Bestehorn H-P, Fischer B, Valina CM, Ferenc M, et al. Impact of the degree of peri-interventional platelet inhibition after loading with clopidogrel on early clinical outcome of elective coronary stent placement. J Am Coll Cardiol. 2006;48(9):1742–50.View ArticlePubMedGoogle Scholar
- Buonamici P, Marcucci R, Migliorini A, Gensini GF, Santini A, Paniccia R, et al. Impact of platelet reactivity after clopidogrel administration on drug-eluting stent thrombosis. J Am Coll Cardiol. 2007;49(24):2312–7.View ArticlePubMedGoogle Scholar
- Marcucci R, Gori AM, Paniccia R, Giusti B, Valente S, Giglioli C, et al. Cardiovascular death and nonfatal myocardial infarction in acute coronary syndrome patients receiving coronary stenting are predicted by residual platelet reactivity to ADP detected by a point-of-care assay: a 12-month follow-up. Circulation. 2009;119(2):237–42.View ArticlePubMedGoogle Scholar
- Campo G, Parrinello G, Ferraresi P, Lunghi B, Tebaldi M, Miccoli M, et al. Prospective evaluation of on-clopidogrel platelet reactivity over time in patients treated with percutaneous coronary intervention relationship with gene polymorphisms and clinical outcome. J Am Coll Cardiol. 2011;57(25):2474–83.View ArticlePubMedGoogle Scholar
- Morel O, El Ghannudi S, Jesel L, Radulescu B, Meyer N, Wiesel M-L, et al. Cardiovascular mortality in chronic kidney disease patients undergoing percutaneous coronary intervention is mainly related to impaired P2Y12 inhibition by clopidogrel. J Am Coll Cardiol. 2011;57(4):399–408.View ArticlePubMedGoogle Scholar
- Muller C, Caillard S, Jesel L, Ghannudi El S, Ohlmann P, Sauleau E, et al. Association of estimated GFR with platelet inhibition in patients treated with clopidogrel. Am J Kidney Dis. 2012;59(6):777–85.Google Scholar
- Collins AJ, Foley RN, Herzog C, Chavers B, Gilbertson D, Herzog C, et al. US renal data system 2012 annual data report. Am J Kidney Dis. 2013;61(1 Suppl 1):A7. –e1–476.View ArticlePubMedGoogle Scholar
- Williams JM, Tuttle-Newhall JE, Schnitzler M, Dzebisashvili N, Xiao H, Axelrod D, et al. Clopidogrel use as a risk factor for poor outcomes after kidney transplantation. Am J Surg. 2014;208(4):556–62.View ArticlePubMedGoogle Scholar
- Sahin G, Akay OM, Bal C, Yalcin AU, Gulbas Z. The effect of calcineurin inhibitors on endothelial and platelet function in renal transplant patients. Clin Nephrol. 2011;76(3):218–25.PubMedGoogle Scholar
- Graff J, Harder S, Wahl O, Scheuermann E-H, Gossmann J. Anti-inflammatory effects of clopidogrel intake in renal transplant patients: effects on platelet-leukocyte interactions, platelet CD40 ligand expression, and proinflammatory biomarkers. Clin Pharmacol Ther. 2005;78(5):468–76.View ArticlePubMedGoogle Scholar
- Aleil B, Ravanat C, Cazenave JP, Rochoux G, Heitz A, Gachet C. Flow cytometric analysis of intraplatelet VASP phosphorylation for the detection of clopidogrel resistance in patients with ischemic cardiovascular diseases. J Thromb Haemost. 2005;3(1):85–92.View ArticlePubMedGoogle Scholar
- Ghannudi SE, Ohlmann P, Meyer N, Wiesel ML, Radulescu B, Chauvin M, et al. Impact of P2Y12 inhibition by clopidogrel on cardiovascular mortality in unselected patients treated by percutaneous coronary angioplasty. JCIN Elsevier Inc. 2010;3(6):648–56.Google Scholar
- Shiraishi J, Kohno Y, Nakamura T, Yanagiuchi T, Hashimoto S, Ito D, et al. Prognostic impact of chronic kidney disease and anemia at admission on in-hospital outcomes after primary percutaneous coronary intervention for acute myocardial infarction. Int Heart J. 2014;55(4):301–6.View ArticlePubMedGoogle Scholar
- Mangiacapra F, Cavallari I, Barbato E, Ricottini E, Patti G, Vizzi V, et al. Impact of chronic kidney disease on platelet reactivity and outcomes of patients receiving clopidogrel and undergoing percutaneous coronary intervention. Am J Cardiol. 2014;113(7):1124–9.View ArticlePubMedGoogle Scholar
- Bae EH, Lim SY, Cho KH, Choi JS, Kim CS, Park JW, et al. GFR and cardiovascular outcomes after acute myocardial infarction: results from the Korea Acute Myocardial Infarction Registry. Am J Kidney Dis. 2012;59(6):795–802.View ArticlePubMedGoogle Scholar
- Alexopoulos D, Xanthopoulou I, Tsigkas G, Damelou A, Theodoropoulos KC, Kassimis G, et al. Predictors of high on-treatment platelet reactivity early after clopidogrel loading in ST-elevation myocardial infarction. Circ J. 2012;76(9):2183–7.View ArticlePubMedGoogle Scholar
- Htun P, Fateh-Moghadam S, Bischofs C, Banya W, Muller K, Bigalke B, et al. Low responsiveness to clopidogrel increases risk among CKD patients undergoing coronary intervention. J Am Soc Nephrol. 2011;22(4):627–33.View ArticlePubMedPubMed CentralGoogle Scholar
- Sibbing D, von Beckerath O, Schömig A, Kastrati A, von Beckerath N. Impact of body mass index on platelet aggregation after administration of a high loading dose of 600 mg of clopidogrel before percutaneous coronary intervention. AJC. 2007;100(2):203–5.View ArticleGoogle Scholar
- Morel O, El Ghannudi S, Hess S, Reydel A, Crimizade U, Jesel L, et al. The extent of P2Y12 inhibition by clopidogrel in diabetes mellitus patients with acute coronary syndrome is not related to glycaemic control: roles of white blood cell count and body weight. Thromb Haemost. 2012;108(2):338–48.View ArticlePubMedGoogle Scholar
- Ojo AO. Cardiovascular complications after renal transplantation and their prevention. Transplantation. 2006;82(5):603–11.View ArticlePubMedGoogle Scholar
- Vanrenterghem YFC, Claes K, Montagnino G, Fieuws S, Maes B, Villa M, et al. Risk factors for cardiovascular events after successful renal transplantation. Transplantation. 2008;85(2):209–16.View ArticlePubMedGoogle Scholar
- Matas AJ, Gillingham KJ, Humar A, Kandaswamy R, Sutherland DER, Payne WD, et al. 2202 kidney transplant recipients with 10 years of graft function: what happens next? Am J Transplant. 2008;8(11):2410–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Aakhus S, Dahl K, Widerøe TE. Cardiovascular morbidity and risk factors in renal transplant patients. Nephrol Dial Transplant. 1999;14(3):648–54.View ArticlePubMedGoogle Scholar
- Graff J, Klinkhardt U, Harder S, Wegert W, Lenz T, Scheuermann E-H, et al. Immunosuppressive therapy regimen and platelet activation in renal transplant patients. Clin Pharmacol Ther. 2002;72(4):411–8.View ArticlePubMedGoogle Scholar
- Siller-Matula JM, Trenk D, Schrör K, Gawaz M, Kristensen SD, Storey RF, et al. How to improve the concept of individualised antiplatelet therapy with P2Y12 receptor inhibitors--is an algorithm the answer? Thromb Haemost. 2015;113(1):37–52.View ArticlePubMedGoogle Scholar
- Siller-Matula JM, Trenk D, Krähenbühl S, Michelson AD, Delle-Karth G. Clinical implications of drug-drug interactions with P2Y12 receptor inhibitors. J Thromb Haemost. 2014;12(1):2–13.View ArticlePubMedGoogle Scholar
- James S, Budaj A, Aylward P, Buck KK, Cannon CP, Cornel JH, et al. Ticagrelor versus clopidogrel in acute coronary syndromes in relation to renal function: results from the Platelet Inhibition and Patient Outcomes (PLATO) Trial. Circulation. 2010;122(11):1056–67.View ArticlePubMedGoogle Scholar