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A case of minimal change disease after the administration of anti receptor activator of nuclear factor kappa B ligand (RANKL) monoclonal antibody: a case report

Abstract

Background

Minimal change disease (MCD) is one of the causes of idiopathic nephrotic syndrome in adults. The pathogenesis of proteinuria in MCD has not been fully understood. Recently, it has been reported that the receptor activator of nuclear factor-kappa B (RANK)/RANK ligand (RANKL) may contribute to the podocyte biology in kidney diseases. Denosumab is a human anti-RANKL monoclonal antibody used to treat osteoporosis. Here we report a case of MCD after denosumab administration.

Case presentation

A 59-year-old male without any episodes of proteinuria was given denosumab to treat osteoporosis. Two weeks after its administration, he noticed a foamy urine and bilateral pretibial edema. Laboratory tests revealed that he had severe proteinuria (15g/g Cr), hypoproteinemia (4.0g/dL), and hypoalbuminemia (1.5g/dL). Based on the results, he was diagnosed with nephrotic syndrome. The proteinuria selectivity index was 0.05, indicating selective proteinuria. Renal biopsy showed minor glomerular abnormality with less tubulointerstitial damage, and electron microscopy showed extensive foot process effacement, indicating MCD. With all these results, glucocorticoid therapy of 50mg/day prednisolone was started. After 4weeks of treatment, the urinary protein level remains high (3.1g/g Cr). Prednisolone therapy was continued, and the levels of proteinuria decreased gradually to the range of partial remission (1.2g/g Cr) with another 7weeks of prednisolone treatment, but complete remission was not achieved.

Conclusions

This might be a case wherein RANKL inhibition is associated with the pathogenesis of MCD. Further studies will be needed to elucidate the causal relationship of RANK-RANKL signaling to the pathogenesis of MCD.

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Background

Minimal change disease (MCD) is one of the causes of idiopathic nephrotic syndrome in adults, accounting for approximately 10–15% of all cases [1, 2]. Most of them respond to steroid treatment, so it has been called steroid-sensitive nephrotic syndrome. However, the response rate in adults is lower, with 5–30% of MCD adult patients not responding to initial steroid therapy [3, 4]. The pathogenesis of proteinuria in MCD has not been fully understood. Recently, it has been reported that podocytes play a key role in the mechanism of proteinuria.

The receptor activator of nuclear factor-kappa B (RANK) and its ligand RANKL are important regulators of bone mineral density [5]. RANK/RANKL is expressed not only in bone marrow-derived cells but also in non-bone marrow-derived cells such as skin epithelial cells, mammary epithelial cells, and renal glomeruli [6,7,8,9,10]. However, the precise role of RANK/RANKL signaling has not been known in proteinuric kidney diseases.

Denosumab is a human anti-RANKL monoclonal antibody used to treat osteoporosis [11]. Here we report a case of MCD after denosumab administration. MCD has not been reported as a side effect of denosumab.

Case presentation

A 59-year-old male with a history of dyslipidemia and without any episodes of proteinuria was diagnosed with osteoporosis when he had knee bone fracture, the cause of which was suspected to be hypogonadotropic hypogonadism due to lower levels of luteinizing hormone, follicle-stimulating hormone, and testosterone. Other pituitary hormones were normal, with the magnetic resonance imaging showing a normal pituitary gland. He did not want to receive androgen replacement therapy, so denosumab with eldecalcitol was given to treat osteoporosis. Two weeks after its administration, he noticed a foamy urine and bilateral pretibial edema, which did not improve spontaneously. Two weeks later, he was admitted to the previous hospital, and laboratory tests showed that he had severe proteinuria (15g/g Cr) and hypoalbuminemia (1.5g/dL). Based on the results, he was diagnosed with nephrotic syndrome. He was then referred to our hospital for further examination and treatment.

Upon admission to our hospital, his height and body weight were 170cm and 65.0kg, respectively, and his blood pressure, heart rate, and body temperature were 150/86mmHg, 90bpm, and 36.3°C, respectively. Physical examination revealed bilateral pretibial pitting edema. Laboratory data for urine tests were as follows (normal ranges in parentheses): protein levels 11.7g/g Cr (<0.15), occult blood 3+ (−), red blood cells 10–19 /high power field (<5). The proteinuria selectivity index was 0.05, indicating selective proteinuria. Serum examination findings were as follows (normal ranges in parentheses): urea nitrogen 15mg/dL (7–23), serum creatinine 0.61mg/dL (0.6–1.0), total protein 4.0g/dL (6.7–8.3), serum albumin 1.5g/dL (4.0–5.0), total cholesterol 376mg/dL (128–219), triglyceride 277mg/dL (30–149), HDL cholesterol 50mg/dL (40–99), LDL cholesterol 271mg/dL (40–119), C-reactive protein 1.0mg/dL (<0.3), IgG 453mg/dL (870–1700), IgA 299mg/dL (110–410), IgM 67mg/dL (33–190), and IgE 147IU/mL (<250). Antinuclear antibody, PR3-ANCA, MPO-ANCA, and anti-GBM antibody were not detected (Table 1). Chest X-ray showed neither cardiac dilatation, pleural fluid nor abnormal shadow. The electrocardiogram showed a left axis deviation. Computed tomography showed a normal-sized kidney with a smooth surface and no mass. Gastrointestinal endoscopy showed no tumor lesion.

Table 1 Laboratory data on admission

Upon admission, we started telmisartan treatment (40mg/day), but the level of proteinuria was not reduced. On the 7th day of admission, renal biopsy was performed to identify the cause of nephrotic syndrome. Light microscopy showed minor glomerular abnormality with less tubulointerstitial damage (Fig. 1a), immunofluorescence studies showed no deposition (Fig. 1b), and electron microscopy showed extensive foot process effacement (Fig. 1c). From these findings and clinical course, he was diagnosed with MCD. To check the glomerular expression of RANK, we tried immunostaining by anti-RANK antibody. However, it was technically difficult because there was not enough amount of section left for immunostaining.

Fig. 1
figure 1

Histopathological images of renal biopsy. a Light microscopy showed minor glomerular abnormality with less tubulointerstitial damage (PAS staining×400). b Immunofluorescence studies showed no deposition. c Electron microscopy showed extensive foot process effacement

With all these results, glucocorticoid therapy of 50mg/day prednisolone was started. After 4weeks of treatment, the urinary protein level remains high (3.1g/g Cr). Therefore, 150mg/day cyclosporine was added but terminated due to liver damage. Prednisolone therapy was continued, and the levels of proteinuria decreased gradually to the range of partial remission (1.2g/g Cr) with another 7weeks of prednisolone treatment (Fig. 2).

Fig. 2
figure 2

Clinical course of the case. The shaded area on the graph represents normal serum albumin level range

Discussion and conclusions

A 59-year-old male developed nephrotic syndrome within 2weeks after denosumab administration. From the clinical course and renal biopsy, he was diagnosed with MCD. Even with the glucocorticoid treatment, he could not achieve complete remission. We speculated that RANKL inhibition by denosumab might be involved in the onset of steroid-resistant nephrotic syndrome.

RANK and RANKL are important regulators of bone mineral density. RANK/RANKL signaling activates a variety of downstream signaling pathways required for osteoclast development, such as nuclear factor-kappa B (NF-B), mitogen-activated protein kinase, and nuclear factor of activated T cells calcineurin-dependent 1 (NFATc1) [12, 13]. RANK/RANKL is expressed not only in bone marrow-derived cells but also in non-bone marrow-derived cells including podocytes [6,7,8,9,10]. RANK/RANKL expression in podocytes has increased in puromycin aminonucleoside nephrosis (PAN), a rat model of podocyte injury and human kidney diseases, such as IgA nephropathy, membranous nephropathy, and focal segmental glomerulosclerosis (FSGS) [10]. In PAN rat model, the level of apoptosis of podocytes was also increased in RANK knockdown by RANK siRNA and was protected by exogenous RANKL exposure, indicating that RANK/RANKL signaling may function as the survival signal for podocytes in PAN [10]. On the other hand, lithium chloride intervention attenuated urinary protein and histopathological change by reducing the levels of RANK, RANKL, and NF-B [14]. In addition to that, Chen et al. reported that RANK and RANKL were overexpressed in the kidneys of db/db mice, a type 2 diabetic nephropathy model, and irbesartan also attenuated urinary protein by downregulating RANK/RANKL signaling and downstream NF-B pathway [15]. In this case, we tried immunostaining by anti-RANK antibody to elucidate the contribution of RANK/RANKL signaling. However, it was technically difficult because there was not enough amount of section left for immunostaining. With these results, RANK/RANKL signaling may have some contribution to the pathogenesis of proteinuric kidney diseases including MCD. To clarify the precise mechanisms of RANK/RANKL signaling in kidney diseases, further studies will be required. Furthermore, regular follow-up of urine test may be needed when denosumab is administered for osteoporosis in patients with existing renal disease and CKD.

As described above, this case showed sustained proteinuria even after steroid and cyclosporine treatment. In a previous report, the observed median T max was 10days, and T 1/2 was 25.4days following the administration of 60mg denosumab [16,17,18]. Therefore, the extended half-life of denosumab might cause a continuous decline of survival signal in podocytes, resulting in persistent proteinuria in this case. Additionally, because it is sometimes difficult to distinguish between MCD and FSGS, this case may have been FSGS. The accumulation of cases of nephrotic syndrome after denosumab treatment will be required to evaluate the association of RANK/RANKL signaling with treatment resistance in nephrotic syndrome.

In summary, the case of MCD after denosumab administration might be associated with RANKL inhibition. Further studies will be needed to elucidate the causal relationship of RANK/RANKL signaling and the pathogenesis of MCD.

Availability of data and materials

Not applicable.

Abbreviations

MCD:

Minimal change disease

RANK:

Receptor activator of nuclear factor-kappa B

RANKL:

Receptor activator of nuclear factor-kappa B ligand

NF- 휅B:

Nuclear factor- kappa B

NFATc1:

Nuclear factor of activated T cells calcineurin-dependent 1

PAN:

Puromycin aminonucleoside nephrosis

FSGS:

Focal segmental glomerulosclerosis

References

  1. Vivarelli M, Massella L, Ruggiero B, Emma F. Minimal change disease. Clin J Am Soc Nephrol. 2017;12:332–45.

    Article  CAS  Google Scholar 

  2. Cameron JS. The nephrotic syndrome and its complications. Am J Kidney Dis. 1987;10:157–71.

    Article  CAS  Google Scholar 

  3. Zhao L, Cheng J, Zhou J, Wu C, Chen J. Enhanced steroid therapy in adult minimal change Nephrotic syndrome: a systematic review and meta-analysis. Intern Med. 2015;54:2101–8.

    Article  CAS  Google Scholar 

  4. Nakayama M, Katafuchi R, Yanase T, Ikeda K, Tanaka H, Fujimi S. Steroid responsiveness and frequency of relapse in adult-onset minimal change nephrotic syndrome. Am J Kidney Dis. 2002;39:503–12.

    Article  CAS  Google Scholar 

  5. Yasuda H, Shima N, Nakagawa N, et al. Osteoclast differentiation factor is a ligand for osteoprotegerin/osteoclastogenesis-inhibitory factor and identical to TRANCE/RANKL. Proc Natl Acad Sci U S A. 1998;95:3597–602.

    Article  CAS  Google Scholar 

  6. Duheron V, Hess E, Duval M, et al. Receptor activator of NF-kappa B (RANK) stimulates the proliferation of epithelial cells of the epidermo-pilosebaceous unit. Proc Natl Acad Sci U S A. 2011;108:5342–7.

    Article  CAS  Google Scholar 

  7. Fata JE, Kong YY, Li J, et al. The osteoclast differentiation factor osteoprotegerin-ligand is essential for mammary gland development. Cell. 2000;103:41–50.

    Article  CAS  Google Scholar 

  8. Beleut M, Rajaram RD, Caikovski M, et al. Two distinct mechanisms underlie progesterone-induced proliferation in the mammary gland. Proc Natl Acad Sci US. 2010;107:2989–94.

    Article  CAS  Google Scholar 

  9. Kartsogiannis V, Zhou H, Horwood NJ, et al. Localization of RANKL (receptor activator of NF kappa B ligand) mRNA and protein in skeletal and extraskeletal tissues. Bone. 1999;25:525–34.

    Article  CAS  Google Scholar 

  10. Liu S, Shi W, Xiao H, et al. Receptor activator of NF-kappaB and podocytes: towards a function of a novel receptor-ligand pair in the survival response of podocyte injury. PLoS One. 2012;7:e41331.

    Article  CAS  Google Scholar 

  11. Bekker PJ, Holloway DL, Rasmussen AS, et al. A single-dose placebo-controlled study of AMG 162, a fully human monoclonal antibody to RANKL, in postmenopausal women. J Bone Miner Res. 2004;19:1059–66.

    Article  CAS  Google Scholar 

  12. Wada T, Nakashima T, Hiroshi N, Penninger JM. RANKL-RANK signaling in osteoclastogenesis and bone disease. Trends Mol Med. 2006;12:17–25.

    Article  CAS  Google Scholar 

  13. Boyce BF, Xing L. Biology of RANK, RANKL, and osteoprotegerin. Arthritis Res Ther. 2007;9(Suppl 1):S1.

    Article  Google Scholar 

  14. Zhou YX, Shi LX, Yang H, et al. Effects of a GSK- 3β inhibitor on the renal expression levels of RANK, RANKL and NF- 휅B in a rat model of diabetic nephropathy. Exp Ther Med. 2016;11:2495–502.

    Article  CAS  Google Scholar 

  15. Chen XW, Du XY, Wang YX, et al. Irbesartan ameliorates diabetic nephropathy by suppressing the RANKL-RANK-NF- 휅B pathway in type 2 diabetic db/db mice. Mediat Inflamm. 2016. https://doi.org/10.1155/2016/1405924.

  16. Chen Q, Hu C, Liu Y, et al. Pharmacokinetics, pharmacodynamics, safety, and tolerability of single-dose denosumab in healthy Chinese volunteers: a randomized, single-blind, placebo-controlled study. PLoS One. 2018;13:e0197984.

    Article  Google Scholar 

  17. Prolia (denosumab) injection [prescribing information]. Amgen Inc, Thousand Oaks. 2015. http://www.accessdata.fda.gov/drugsatfda_docs/label/2015/125320s174lbl.pdf. Accessed 25 Jan 2016.

  18. Xgeva (denosumab) injection [prescribing information]. Amgen Inc, Thousand Oaks. 2015. http://www.accessdata.fda.gov/drugsatfda_docs/label/2015/125320s168lbl.pdf. Accessed 25 Jan 2016.

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All authors made substantial contributions to the acquisition and interpretation of data, critical revision of the manuscript for important intellectual content, and approved the final version for publication. KH, NS and TW made substantial contributions to the conception of this report. NY, HO, KS, TM, SK, TT, AH, YI, MS, KF performed the treatment. All authors read and approved the final manuscript.

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Correspondence to Norihiko Sakai.

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Horikoshi, K., Sakai, N., Yamamoto, N. et al. A case of minimal change disease after the administration of anti receptor activator of nuclear factor kappa B ligand (RANKL) monoclonal antibody: a case report. BMC Nephrol 21, 416 (2020). https://doi.org/10.1186/s12882-020-02066-3

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