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Granulomatous interstitial nephritis with CTLA-4 haploinsufficiency: a case report



Cytotoxic T lymphocyte antigen-4 (CTLA-4) is an essential inhibitory regulator of immune activation. CTLA-4 haploinsufficiency is known to be associated with dysregulation of FOXP3+ regulatory T cells, hyperactivation of effector T cells, and lymphocytic infiltration of multiple organs. However, there have only been a few reports of renal involvement with CTLA-4. Herein, we present a case of acute granulomatous tubulointerstitial nephritis (TIN) in a patient with CTLA-4 haploinsufficiency.

Case presentation

A 44-year-old man presented with a 3-week history of fever and malaise, and subsequently developed acute kidney injury (AKI) a few days after treatment with levofloxacin (LVFX). A kidney biopsy and immunohistochemical staining revealed granulomatous TIN with dominantly infiltrating CD4+ T cells. General symptoms and renal impairment showed improvement after discontinuation of LVFX and initiation of oral steroids. However, they worsened following steroid tapering. Further, a colon biopsy analysis showed similar findings to the renal tissue analysis. We suspected that granulomatous TIN was possibly associated with CTLA-4 haploinsufficiency. Therefore, the patient was transferred to another hospital for further treatment of CTLA-4 haploinsufficiency using immunosuppressive agents.


There have been few reports regarding renal involvement of CTLA-4 haploinsufficiency. In the present case, granulomatous TIN could have arisen due to instability of immune regulatory functions, such as CTLA-4 haploinsufficiency, and treatment with LVFX could have triggered immunologic activation and severe inflammation as well as renal dysfunction.

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Cytotoxic T lymphocyte antigen-4 (CTLA-4) is an essential inhibitory regulator of immune activation, i.e., a “checkpoint” molecule in immune tolerance [1, 2]. Homozygous deficiency of CTLA4 in mice causes fatal multiorgan lymphocytic infiltration and destruction [3,4,5]; however, the consequences of CTLA4 mutations in humans are unknown. Human CTLA-4 haploinsufficiency is an autosomal dominant immune dysregulation syndrome with a heterozygous germline mutation in CTLA4 that causes dysregulation of FOXP3+ regulatory T cells (Tregs), hyperactivation of effector T cells, and lymphocytic infiltration of multiple organs [6, 7]. Although several clinical symptoms such as autoimmune hemolytic anemia, immune thrombocytopenia, and lymphocytic infiltration in multiple organs have been reported in patients with CTLA-4 haploinsufficiency [6, 7], renal involvement has been rarely reported. Here, we report a case of granulomatous tubulointerstitial nephritis (TIN) with CTLA-4 haploinsufficiency.

Case presentation

A 44-year-old Japanese man presented with fever and malaise to the Department of Hematology at our hospital. He was prescribed medication for the common cold and levofloxacin (LVFX), 500 mg once daily, for a week; however, his fever did not subside. He presented to our hospital again and was prescribed the same medications for an additional week. However, the fever persisted, and the patient experienced loss of appetite. He was then admitted to our hospital for further examination and treatment. The patient had a prior history of immune thrombocytopenia at 19 years of age and of autoimmune hemolytic anemia at 32 years of age. During his late thirties, three of his children showed similar symptoms (Fig. 1a) and genetic testing revealed the same genetic mutation, a heterozygous nonsense p. Q12X mutation in CTLA4, in them. The expression level of reactive CTLA4 mRNA in his two daughters, measured by real-time PCR, was decreased compared to that in the control group (Fig. 1b). Although a PCR test was not performed for the patient, he and his two daughters were diagnosed with CTLA-4 haploinsufficiency due to the presence of the same mutation.

Fig. 1
figure 1

CTLA4 mutation in our patient’s family and decreased CTLA4 mRNA expression in his two daughters. a The family tree shows the same genetic mutation, heterozygous nonsense p. Q12X mutation in CTLA4, found in our patient (blue arrow) and his three children. His wife has wild type with no mutation. Their main clinical symptoms are described. b Real-time PCR using peripheral blood mononuclear cells (PBMCs), after stimulation with anti-CD3 and anti-CD28 obtained from his two daughters, showed decreased expression of the reactive CTLA4 mRNA compared to that in the control group

On physical examination at admission, his temperature was 37.8 °C. The patient’s cardiovascular, pulmonary, abdominal, and neurological examinations were normal, and no edema, arthralgia, swelling, or pain in the cervical lymph nodes were reported.

His laboratory data on admission are described in Table 1. His serum creatinine level was significantly elevated from the baseline level of 0.8 mg/ dL. No occult blood, proteinuria, pyuria, or urinary casts were detected on urinalysis. However, tubular injury markers were elevated. After admission, oral LVFX was discontinued, and piperacillin-tazobactam was initiated to treat the persistent fever. However, the fever did not subside, and serum creatinine levels increased to 4.23 mg/dL on the fourth day of admission. He was then referred to our department.

Table 1 Laboratory findings on admission

The laboratory data on admission are shown. Inflammatory markers were elevated. IgG did not change remarkably compared to baseline levels. Upon urinalysis, occult blood, pyuria, or any casts were not detected; however, mild proteinuria and elevated tubular injury markers were found.

A kidney biopsy was performed on the seventh day. The specimen contained 12 glomeruli, of which one was globally sclerosed Light microscopy showed diffuse interstitial inflammation composed of numerous lymphocytes with foreign body giant cells associated with non-caseating granulomas, few eosinophils, and plasma cells. Tubulitis, with no evidence of vasculitis, was also observed. All other glomeruli, excluding the sclerosed, showed no significant changes (Fig. 2a). Immunofluorescence revealed non-specific findings, and electron microscopy revealed no electron-dense deposits. Ziehl–Neelsen acid-fast staining was negative. Immunohistological staining showed CD3+ and CD4+ T cells dominantly infiltrating the interstitial area (Fig. 2b). Moreover, immunostaining for FOXP3, the master transcription factor of Tregs, showed a decrease in the percentage of FOXP3+ cells within CD4+ T cells was decreased (Fig. 2c).

Fig. 2
figure 2

Pathological findings of renal and colon biopsy. (a-i) Light microscopy of the first renal biopsy at low power showing diffuse interstitial infiltrating of lymphocytes. (a-ii) Numerous lymphocytes with foreign body giant cells associated with non-caseating granulomas and a few eosinophils and plasma cells (yellow arrowheads). (a-iii) Granuloma formation at high power field. b Immunohistochemistry showing a more dominant infiltration of CD3+ and CD4+ T cells in the interstitial area compared to CD8+ and CD20+ T-cells. c FOXP3 staining of the first renal biopsy, wherein the proportion of FOXP3+ cells within CD4+ T cells was not as high. d Improved interstitial inflammation and diminished granuloma in the second renal biopsy compared to that in the first biopsy using light microscopy at low power (d-i), and high power (d-ii). e Dominant CD3+ and CD4+ T cell infiltration in the interstitial area in both the first and second renal biopsy, even though the stained area was decreased in the second biopsy compared with that in the first biopsy. f Colon biopsy showing CD4+ cells as the dominant lymphocytes infiltrating the intestinal mucosa in immunohistological staining, similar to the findings in the kidney

Based on the histopathological findings, the patient was diagnosed with granulomatous TIN. We thus speculated that granulomatous TIN was either associated with the use of LVFX or with the presence of CTLA-4 haploinsufficiency.

Clinical course during hospitalization is shown in Fig. 3. We initiated oral prednisolone (PSL) therapy, 30 mg/day (0.5 mg/kg of body weight). After treatment, the fever subsided, and renal function improved gradually. The serum creatinine level decreased to 1.99 mg/dL within 2 weeks. The patient was discharged on the 17th day, and the dose of PSL was tapered to 25 mg/day. During outpatient follow-up, serum creatinine levels gradually improved, but did not decrease beyond 1.0–1.4 mg/dL even after PSL was tapered to 10 mg/day; moreover, malaise and arthralgia reoccurred. To re-evaluate the pathophysiology, we performed a second biopsy 5 months after the first. The results indicated that interstitial inflammation had improved and granuloma had diminished compared to that in the first biopsy (Fig. 2d). Similar to the first biopsy, CD3+ and CD4+ T cells predominantly infiltrated the interstitial area. However, in all immunohistological staining, the stained area was decreased (Fig. 2e) compared to that in the first biopsy. We evaluated five nonoverlapping interstitial fields in each tissue stained with CD3. CD3 positive areas were measured with the WinRoof Image Analyzer, version 4.3.0 (Mitani, Tokyo, Japan) and we compared the ratio of CD3 positive area relative to the entire area between the first and the second biopsy. As a result, there is significant decrease in the ratio of CD3 positive area in the second biopsy(9.6% ± 2.1) compared with the first(46.8% ± 5.3)(p = 0.043).

Fig. 3
figure 3

Clinical course during hospitalization. Although piperacillin-tazobactam was initiated after admission, the fever did not subside, and serum creatinine levels increased, then he was referred to our department. A kidney biopsy was performed on the 7th hospital day, and oral prednisolone (PSL) therapy, 30 mg (0.5 mg/kg) daily was initiated. After treatment, the fever subsided, and renal function improved gradually. The serum creatinine level decreased to 1.99 mg/dL within 2 weeks. Patient was discharged on the 17th day

A colon biopsy was also performed as the patient reported diarrhea. The results showed lymphocytes infiltrating the intestinal mucosa; furthermore, CD4+ cells were the dominant lymphocytes (Fig. 2f). This was similar to the findings of the first renal biopsy.

The patient was then transferred to the same hospital as his children to receive further therapeutic intervention for CTLA-4 haploinsufficiency. In the other hospital, the patient was treated with abatacept, a recombinant fusion protein comprising the extracellular domain of human CTLA4, which inhibits the activation of T cells. PSL was maintained at 5 mg/day. After stating abatacept, his general symptoms (malaise, arthralgia) significantly improved and his creatinine level stayed around 1.0–1.2 mg/dl without more elevation.

Discussion and conclusions

Here, we present a case of granulomatous TIN in a patient with CTLA-4 haploinsufficiency. It was difficult to clearly distinguish between drug-induced tubulointerstitial nephritis (DI-TIN) and renal involvement due to CTLA-4 haploinsufficiency.

The incidence of granulomatous TIN varied in different regions, ranging from 17.5 to 44.7%, and was most commonly caused due to exposure to drugs such as antibiotics [8,9,10,11]. In many case reports, granulomatous TIN has been attributed to the intake of penicillin and cephalosporins. Fluoroquinolones have been rarely reported to cause granulomatous TIN [12].

DI-TIN typically occurs within 3 weeks of initiating therapy with the causative drug in 80% of cases, with an average delay of 10 days following antibiotic use. It is characterized by mild proteinuria, sterile pyuria, and external manifestations that indicate systemic reactions such as fever, skin rash, and eosinophilia [13]. However, the detailed pathogenesis of DI-TIN, which represents the most common form of acute interstitial nephritis, remains unclear. There is a paucity of data regarding T-lymphocyte subsets that infiltrate the interstitial lesions. Therefore, a distinct diagnosis of DI-TIN seems difficult based on pathological characteristics alone. In our patient, LVFX had been used 2 weeks before the development of AKI. However, fever and elevated levels of inflammation markers were observed before the initiation of LVFX, and the general symptoms worsened following steroid tapering without any additional drug use, which suggested the involvement of other factors aside from LVFX use as the causation of granulomatous TIN.

Patients with CTLA-4 haploinsufficiency are reported to have lymphocytic infiltration of organs and lymphadenopathy. T cell infiltration in multiple organs such as the intestine, lung, bone marrow, and the central nervous system has also been reported [7]. However, renal involvement is very rare and its histological findings are limited [6].

Recently, immune checkpoint inhibitors, which are monoclonal antibodies directed against immune checkpoint proteins, have been increasingly used in the treatment of various cancers. Ipilimumab, an anti-CTLA-4 antibody has been approved for treating unresectable or metastatic melanoma and has shown favorable tumor response and extended patient survival [14]. Immune checkpoint inhibitors also have been known to induce side effects called “immune-related adverse events”. Several reports have described renal disorders related to ipilimumab, which mostly occur as AKI [15,16,17,18,19,20], but rarely as a nephrotic syndrome [21]. Renal biopsy showed acute interstitial nephritis with or without granuloma, and immunohistochemistry showed extensive T cell-dominant interstitial infiltrates that were mainly positive for CD3 and CD4 T cells [19, 20]. These results are similar to those in our patient. It could thus be considered that patients with CTLA4 inhibition caused by ipilimumab show pathological and clinical features almost similar to those of patients suffering from CTLA-4 haploinsufficiency.

In previous reports of heterozygous germline mutations in CTLA4 [6, 7], a high number of CD4+ and CD3+ cells infiltrated several organs, which was consistent with the findings in our patient. It was assumed that Tregs lost their suppressive capacity and that uncontrolled activation of auto-reactive T cells occurred due to CTLA-4 haploinsufficiency and contributed to inflammatory cell infiltration, in this case. These cells then migrated and infiltrated the target tissue via tissue self-antigen-specific T cell activation [22]. However, there are contradicting findings regarding the frequency of FOXP3+ Tregs within CD4+ T cells. Schubert et al. showed increased frequency compared to the control group [7]. On the contrary, Kuehn et al. reported significantly fewer FOXP3+ and CD25+ cells in peripheral blood of patients with CTLA-4 haploinsufficiency than in healthy donors, which were similar to our results, although their results were obtained using peripheral blood [6].

There are few reports of FOXP3 staining on kidney tissue in patients with acute interstitial nephritis as an adverse effect of CTLA-4 inhibitor. In a patient treated with a combination of nivolumab (anti-programmed cell death-1 antibody) and ipilimumab (anti-CTLA-4 antibody), immunohistochemistry showed extensive T cell-dominant infiltrates and FOXP3+ regulatory T cells in the interstitium; however, the frequency was not reported [19].

Therefore, it remains unclear if the degree of FOXP3-positive staining is associated with the function of FOXP3-positive regulatory T cells and the pathology or activity of CTLA-4 haploinsufficiency. It is also assumed that the percentage of FOXP3-positive cells in CD4+ T cells may vary during each inflammatory phase. Unfortunately, we could not quantify FOXP3 levels in renal biopsy or the frequency of Treg cell phenotypes in blood. This is a limitation in this case study and further studies are needed to understand the extent of Treg dysfunction.

In conclusion, we report a very rare case of granulomatous TIN with CTLA-4 haploinsufficiency. In this case, granulomatous TIN was suggested to be more likely associated with CTLA-4 haploinsufficiency, rather than with DI-TIN. Additionally, due to immune regulatory instability caused by CTLA-4 haploinsufficiency, treatment with LVFX could have triggered immunologic activation and severe inflammation as well as renal dysfunction.

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acute kidney injury


cytotoxic T lymphocyte antigen-4


drug-induced tubulointerstitial nephritis


Tubulointerstitial nephritis




peripheral blood mononuclear cells




regulatory T cells


  1. Wing K, Onishi Y, Prieto-Martin P, Yamaguchi T, Miyara M, Fehervari Z, et al. CTLA-4 control over FOXP3+ regulatory T cell function. Science. 2008;322:271–5.

    Article  CAS  PubMed  Google Scholar 

  2. Friedline RH, Brown DS, Nguyen H, Kornfeld H, Lee J, Zhang Y, et al. CD4+ regulatory T cells require CTLA-4 for the maintenance of systemic tolerance. J Exp Med. 2009;206:421–34.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Tivol EA, Borriello F, Schweitzer AN, Lynch WP, Bluestone JA, Sharpe AH. Loss of CTLA-4 leads to massive lymphoproliferation and fatal multiorgan tissue destruction, revealing a critical negative regulatory role of CTLA-4. Immunity. 1995;3:541–7.

    Article  CAS  PubMed  Google Scholar 

  4. Waterhouse P, Penninger JM, Timms E, Wakeham A, Shahinian A, Lee KP, et al. Lymphoproliferative disorders with early lethality in mice deficient in Ctla-4. Science. 1995;270:985–8.

    Article  CAS  PubMed  Google Scholar 

  5. Chambers CA, Sullivan TJ, Allison JP. Lymphoproliferation in CTLA-4-deficient mice is mediated by costimulation-dependent activation of CD4+ T cells. Immunity. 1997;7:885–95.

    Article  CAS  PubMed  Google Scholar 

  6. Kuehn HS, Ouyang W, Lo B, Deenick EK, Niemela JE, Avery DT, et al. Immune dysregulation in human subjects with heterozygous germline mutations in CTLA4. Science. 2014;345:1623–7.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Schubert D, Bode C, Kenefeck R, Hou TZ, Wing JB, Kennedy A, et al. Autosomal dominant immune dysregulation syndrome in humans with CTLA4 mutations. Nat Med. 2014;20:1410–6.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Mignon F, Méry JP, Mougenot B, Ronco P, Roland J, Morel-Maroger L. Granulomatous interstitial nephritis. Adv Nephrol Necker Hosp. 1984;13:219–45.

    CAS  PubMed  Google Scholar 

  9. Viero RM, Cavallo T. Granulomatous interstitial nephritis. Hum Pathol. 1995;26:1347–53.

    Article  CAS  PubMed  Google Scholar 

  10. Bijol V, Mendez GP, Nosé V, Rennke HG. Granulomatous interstitial nephritis: a clinicopathologic study of 46 cases from a single institution. Int J Surg Pathol. 2006;14:57–63.

    Article  PubMed  Google Scholar 

  11. Javaud N, Belenfant X, Stirnemann J, Laederich J, Ziol M, Callard P, et al. Renal granulomatoses: a retrospective study of 40 cases and review of the literature. Medicine. 2007;86:170–80.

    Article  PubMed  Google Scholar 

  12. Ramalakshmi S, Bastacky S, Johnson JP. Levofloxacin-induced granulomatous interstitial nephritis. Am J Kidney Dis. 2003;41:E7.

    Article  PubMed  Google Scholar 

  13. Rossert J. Drug-induced acute interstitial nephritis. Kidney Int. 2001;60:804–17.

    Article  CAS  PubMed  Google Scholar 

  14. Hodi FS, O’Day SJ, McDermott DF, Weber RW, Sosman JA, Haanen JB, et al. Improved survival with ipilimumab in patients with metastatic melanoma. N Engl J Med. 2010;363:711–23.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Izzedine H, Gueutin V, Gharbi C, Mateus C, Robert C, Routier E, et al. Kidney injuries related to ipilimumab. Investig New Drugs. 2014;32:769–73.

    Article  CAS  Google Scholar 

  16. Voskens CJ, Goldinger SM, Loquai C, Robert C, Kaehler KC, Berking C, et al. The price of tumor control: an analysis of rare side effects of anti-CTLA-4 therapy in metastatic melanoma from the ipilimumab network. PLoS One. 2013;8:e53745.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Forde PM, Rock K, Wilson G, O’Byrne KJ. Ipilimumab-induced immune-related renal failure–a case report. Anticancer Res. 2012;32:4607–8.

    PubMed  Google Scholar 

  18. Thajudeen B, Madhrira M, Bracamonte E, Cranmer LD. Ipilimumab granulomatous interstitial nephritis. Am J Ther. 2015;22:e84–7.

    Article  PubMed  Google Scholar 

  19. Murakami N, Borges TJ, Yamashita M, Riella LV. Severe acute interstitial nephritis after combination immune-checkpoint inhibitor therapy for metastatic melanoma. Clin Kidney J. 2016;9:411–7.

    Article  PubMed  PubMed Central  Google Scholar 

  20. Belliere J, Meyer N, Mazieres J, Ollier S, Boulinguez S, Delas A, et al. Acute interstitial nephritis related to immune checkpoint inhibitors. Br J Cancer. 2016;115:1457–61.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Fadel F, El Karoui K, Knebelmann B. Anti-CTLA4 antibody- induced lupus nephritis. N Engl J Med. 2009;361:211–2.

    Article  CAS  PubMed  Google Scholar 

  22. Ise W, Kohyama M, Nutsch KM, Lee HM, Suri A, Unanue ER, et al. CTLA-4 suppresses the pathogenicity of self antigen-specific T cells by cell-intrinsic and cell-extrinsic mechanisms. Nat Immunol. 2010;11:129–35.

    Article  CAS  PubMed  Google Scholar 

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We would like to thank Yoshiaki Shikama from the Department of Infectious diseases and Immunology of Kanagawa Children’s Medical Center for providing information on conducting a genetic analysis of the patient and his family. In addition, we thank Editage ( for English language editing.


The authors did not receive any funding.

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KK wrote and revised the manuscript; TS, SS, AH approved the final version and supervised; KK, KM, MT, and SS actively participated in the treatment of the patient. All authors read and approved the final manuscript.

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Correspondence to Sayuri Shirai.

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Kohatsu, K., Suzuki, T., Takimoto, M. et al. Granulomatous interstitial nephritis with CTLA-4 haploinsufficiency: a case report. BMC Nephrol 23, 367 (2022).

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  • CTLA-4 haploinsufficiency
  • Drug-induced interstitial nephritis
  • Granuloma
  • Acute kidney injury
  • Case report