Skip to main content
Download PDF

Effect of glycemic index and carbohydrate intake on kidney function in healthy adults

BMC Nephrology volume 17, Article number: 70 (2016) Cite this article

Abstract

Background

Replacing carbohydrate with protein acutely increases glomerular filtration rate (GFR) but is associated with faster, long-term kidney disease progression. The effects of carbohydrate type (i.e. glycemic index, GI) on kidney function are unknown.

Methods

We conducted an ancillary study of a randomized, crossover feeding trial in overweight/obese adults without diabetes or kidney disease (N = 163). Participants were fed each of four healthy, DASH-like diets for 5 weeks, separated by 2-week washout periods. Weight was kept constant. The four diets were: high GI (GI ≥65) with high %carb (58 % kcal) (reference diet), low GI (≤45) with low %carb (40 % kcal), low GI with high %carb; and high GI with low %carb. Plasma was collected at baseline and after each feeding period. Study outcomes were cystatin C, β2-microglobulin (β2M), and estimated GFR based on cystatin C (eGFRcys).

Results

Mean (SD) age was 52 (11) years; 52 % were women; 50 % were black. At baseline, mean (SD) cystatin C, β2M, and eGFRcys were 0.8 (0.1) mg/L, 1.9 (0.4) mg/L, and 104 (16) mL/min/1.73 m2. Compared to the high GI/high %carb diet, reducing GI, %carb, or both increased eGFRcys by 1.9 mL/min/1.73 m2 (95 % CI: 1.1, 2.7; P < 0.001), 3.0 mL/min/1.73 m2 (1.9, 4.0; P < 0.001), and 4.5 mL/min/1.73 m2 (3.5, 5.4; P < 0.001), respectively. Increases in eGFRcys from reducing GI were significantly associated with increases in eGFRcys from reducing %carb (P < 0.001). Results for cystatin C and β2M reflected eGFRcys.

Conclusions

Reducing GI increased GFR. Reducing %carb by increasing calories from protein and fat, also increased GFR. Future studies on GI should examine the long-term effects of this increase in GFR on kidney injury markers and clinical outcomes.

Trial registration

Clinical Trials.gov, number: NCT00608049 (first registered January 23, 2008)

Peer Review reports

Background

Replacing dietary carbohydrates with protein increases glomerular filtration rate (GFR) acutely and is associated with faster chronic kidney disease (CKD) progression over the long-term [1–3]. Whether carbohydrate quality affects GFR is unknown. Glycemic index (GI) is a measure of carbohydrate quality, i.e. the amount of glucose released into circulation from carbohydrate-based foods [4]. GI is determined by comparing the area under a 2-h glucose curve measured after consumption of a standardized amount of carbohydrates from two food items (the food of interest and a reference food) [5]. Previous observational studies demonstrated that higher GI is associated with prevalent CKD [6]. However, this association has not been examined in a trial setting and could have implications for CKD prevention.

In this analysis of data from “The Effect of Amount and Type of Dietary Carbohydrates on Risk for Cardiovascular Heart Disease and Diabetes Study” (OMNICARB), we determined the effects of reducing GI or the proportion of carbohydrates (%carb) on GFR in relatively healthy adults, using a cystatin C estimating equation. We further estimated GFR with a creatinine-based equation for comparison. We hypothesized that reducing GI would increase GFR as a result of metabolic changes, and that reducing %carb (i.e. increasing the proportion of protein) would increase GFR. We reported this relationship to change in protein intake in an earlier publication from a different feeding study (OMNI-HEART) [7]. A cystatin C-based equation, rather than a creatinine-based equation, was our primary outcome because of a concern that changes in dietary protein intake would influence serum creatinine levels and interfere with accurate estimation of kidney function [7].

Methods

The rationale, design, and main effects of OMNICARB were described previously [8]. The current ancillary study utilized stored plasma specimens to examine the effects of amount and type of carbohydrates on markers of kidney function.

OMNICARB was a randomized, crossover trial, comprising 4-dietary interventions: high GI (GI ≥65) with high %carb (58 % kcal from carbohydrates) (reference diet), low GI (GI ≤45) with low %carb (40 % kcal from carbohydrates), low GI with high %carb; and high GI with low %carb [8]. Other key nutrients were similar across the four diets (Table 1). By design, the diets were healthy using the core nutrient profile of the DASH diet [9]. Each diet was isocaloric, was prepared with commonly available foods, and was low in saturated fat, cholesterol, and sodium, but rich in fruits, vegetables, fiber, potassium, and other minerals. Note that the low %carb diets were also higher in % protein.

Table 1 Nutrient composition of the four diets used in OmniCarba
Full size table

Participant recruitment

Trial participants were adult men and women, residing in and around Boston, Massachusetts, and Baltimore, Maryland. Participants were ≥30 years, had a body mass index ≥25 kg/m2 with a systolic BP of 120–159 and a diastolic BP <100 mmHg. Persons with a prior diagnosis of diabetes or cardiovascular disease were excluded [8]. Persons were also excluded if they had renal insufficiency on screening labs, based on an estimated GFR <40 ml/min per 1.73 m2. Similarly, participants were deemed eligible for inclusion if baseline systolic blood pressure was 120–159 mmHg and diastolic blood pressure was <100 mmHg (mean over three screening visits). Anyone with stage 2 hypertension (systolic blood pressure > 160 or diastolic blood pressure > 100 mmHg) based on the mean of three screening visits were excluded. Anyone with a mean systolic blood pressure >170 or diastolic blood pressure >105 mmHg at any one visit were also excluded [8]. Institutional Review Boards approved the study protocol, and all participants provided written informed consent.

Controlled feeding

Controlled feeding began in August 2008 and was completed in December 2010. Participants were randomly assigned to 1 of 8 dietary sequences of the 4 diets [8]. Feeding periods lasted for 5 weeks, separated by 2-week washout periods. Calorie targets were determined for each participant based on body size, sex, and physical activity level. Participants were provided all of their foods and caloric intake was adjusted to maintain weight within 2 % of participants’ baseline values. Participants were encouraged to maintain the same activity levels and alcohol consumption throughout the study. Every day, participants ate at least one meal at the study site and meal attendance and meal consumption were monitored. Participants also completed a diary in which they listed any consumption of non-protocol foods. Biochemical markers of dietary compliance were urine urea nitrogen, urine creatinine, urine sodium, and urine potassium.

Measurement of outcomes

Laboratory specimens were collected for each participant at baseline and at the completion of each 5-week feeding period. Cystatin C, β2 microglobulin (β2M), and creatinine were measured in plasma samples that were collected in tubes, centrifuged, and stored at −70 °C. Cystatin C and β2M were used to estimate kidney function, whereas plasma creatinine and estimated glomerular filtration rate based on creatinine (eGFRcreat) were measured for purposes of comparison. When compared to creatinine, these markers are less influenced by factors know to affect creatinine such as race [10] and diet (e.g. protein intake) [7]. Both cystatin C and β2M were measured with particle-enhanced immunonephelometric assays (N Latex Cystatin C assay and N Latex β2M assay, Dade Behring, IL). Inter-assay coefficients of variation for cystatin C and β2M were 2.0 % (mean 0.917 mg/L) and 1.8 % (mean 2.184 mg/L), respectively. Creatinine was measured in stored plasma specimens using standardized laboratory assays with an inter-assay coefficient of variation of 6.6 % (mean 0.653 mg/dL). Glomerular filtration rate was estimated using the Chronic Kidney Disease Epidemiology Collaboration cystatin C-based equation (eGFRcys) [11] and the Chronic Kidney Disease Epidemiology Collaboration creatinine-based GFR equation (eGFRcreat) [12]. As opposed to other estimating equations, these equations were used because they were developed in a population sample that included individuals, who like the participants of the OMNICARB trial, did not have chronic kidney disease (CKD).

Other covariate measurements and definitions

Additional laboratory and physical exam covariates were determined as follows. Body mass index (BMI) was calculated using baseline height and weight measurements and was dichotomized as 25–29.9 kg/m2 or ≥30 kg/m2. Waist circumference (cm) was measured at the level of the umbilicus. Fasting glucose and insulin were measured at baseline and at the end of each feeding period. The homeostasis model assessment index (HOMA) was calculated using HOMA = [(fasting serum insulin concentration in μU/mL) x (fasting serum glucose concentration in mmol/L)]/22.5 and dichotomized based on the baseline median value of ≥1.48. Similarly, traditional assays were used to measure total triglycerides and HDL cholesterol. LDL cholesterol levels were estimated by the Friedewald equation [13]. Triglycerides were dichotomized using the baseline median value of 83.8 mg/dL. Uric acid and high sensitivity C-reactive protein were also measured in plasma collected at baseline and the end of each feeding period.

Baseline hypertension status (yes or no) was determined by an average of 3 baseline blood pressure measurements in which systolic blood pressure was >140 mmHg or diastolic blood pressure was >90 mmHg. No participants were taking antihypertensive medications. Blood pressure was also measured at the end of each feeding period as the average of 3 measurements by trained and certified staff using a validated automated oscillometric OMRON 907 device.

Mediators of glomerular filtration

At baseline and at the end of each feeding period, we assessed potential mediators of glomerular filtration, including markers of glucose homeostasis (fasting glucose, insulin, triglycerides), endothelial function (uric acid, systolic BP, diastolic BP), and inflammation (high sensitivity C-reactive protein).

Statistical analysis

The primary outcomes examined in this study were cystatin C, β2M, and eGFRcys. We estimated change from baseline for each of the 4 diets. Using the high %carb, high GI diet as a reference, we then calculated the effects of reducing GI alone, %carb alone, or both GI and %carb for each outcome and generated forest plots for visual representation. In addition, we performed stratified analyses by covariates known to be associated with insulin resistance, namely, race (non-Hispanic black versus white), baseline hypertension status, baseline triglycerides, baseline BMI, and baseline HOMA. All of the above comparisons were performed via generalized estimating equation (GEE) regression models, using a Huber and White robust variance estimator [14], which assumed an exchangeable working correlation matrix. P-values for each stratum were generated using interaction terms. The above analyses were repeated for creatinine and eGFRcreat for comparison.

We also used linear regression to determine whether the change in eGFRcys which resulted from reducing GI was associated with change in eGFRcys which resulted from reducing %carb. A positive association would suggest a common mechanism of action for GI and %carb on kidney function. We further performed a mediation analysis of eGFRcys, adjusting for concurrent changes in markers of glucose homeostasis (fasting glucose, insulin, triglycerides), endothelial function (uric acid, systolic blood pressure, diastolic blood pressure), and inflammation (high sensitivity C-reactive protein), all thought to influence GFR.

For dietary compliance measures, namely, urine urea nitrogen, urine creatinine, urine sodium, and urine potassium, we performed baseline and between-diet comparisons only, using similar models described above. A sensitivity analysis using feeding period 1 alone was conducted to eliminate potential carryover effects. We also performed a sensitivity analysis examining every possible dietary comparison grouped by GI, %carb, or simultaneous changes in both GI and %carb. The purpose of this analysis was to confirm our primary comparison between diets, which utilized the high GI, high %carb diet as our reference group. All analyses were performed in STATA version 11.1 (Stata Corporation, College Station, TX, USA). Statistical significance was defined as P ≤ 0.05. Missing data specimens were excluded from analyses as they were few (N = 4) and evenly distributed between feeding periods and diets.

Results

Baseline characteristics of the randomized, study population are shown in Table 2. Overall mean age was 52.1 ± 11.4 years; 52 % of the participants were women, and 50 % were non-Hispanic blacks. Furthermore, 56 % of participants were obese, and 26 % had hypertension.

Table 2 Baseline characteristics of trial participants (N = 163), mean ± SD or No. (%)
Full size table

End-of-period measures were compared to baseline measures (Table 3). Cystatin C was significantly greater during the high GI, high %carb diet (P =0.04) and significantly lower during the high GI/low %carb, and low GI/low %carb diets (both P ≤0.001). β2M was significantly lower during the low GI/low %carb diet (P =0.002). While eGFRcys decreased significantly ~1 mL/min/1.73 m2 during the high GI/high %carb diet (P =0.02), it increased significantly during the high GI/low %carb (~2 mL/min/1.73 m2; P =0.002) and low GI/low %carb diets (~3 mL/min/1.73 m2; P <0.001). Creatinine did not change significantly during any dietary period throughout the trial. There was no significant difference between end-of-period eGFRcreat and baseline during any of the four diets.

Table 3 Plasma markers of kidney function and urine markers of compliance, N = 159
Full size table

When compared with baseline, urine urea nitrogen was significantly higher during the low %carb diets (P <0.001 for high GI/low %carb and P <0.001 for low GI/low %carb) consistent with the higher protein composition of these diets. Urine creatinine tended to decrease during the high %carb diets (P =0.12 for high GI/high %carb and P =0.01 for low GI/high %carb), consistent with the diets’ lower protein composition (16 % vs. 23 %). For all diets, urine sodium significantly decreased and urine potassium increased (all P-values <0.001). This was expected given the low sodium and high fruits and vegetable-based foods inherent in all the OMNICARB diets.

The effects of reducing GI or %carb on β2M, cystatin C, and eGFRcys were compared across diets, using the high GI,/high %carb diet as a reference (Fig. 1a-c). Reducing GI, %carb, or both GI and %carb significantly reduced cystatin C (P <0.001 for all three) and β2M (P <0.001 for all three). Each of the diets significantly increased eGFRcys (P <0.001 for all three). Furthermore, the magnitude of effect was higher, ranging from ~2 mL/min/1.73 m2 (reduced GI) and ~3 mL/min/1.73 m2 (reduced %carb) to 4.5 mL/min/1.73 m2, when both GI and %carb were reduced.

Fig. 1
figure 1

The effects (95 % confidence intervals) of reducing glycemic index (GI), reducing the proportion of carbohydrates (%carb), or reducing both GI and %carb on: a cystatin C (mg/L), b β2-microglobulin (mg/L), and c cystatin C-based estimated glomerular filtration rate (eGFRcys) (mL/min/1.73 m2) measured at the end of each feeding period. The reference diet was the high GI/high %carb diet

Full size image

The effects of dietary carbohydrates on plasma creatinine and eGFRcreat were also assessed (Additional file 1: Figure S1A-B). While reducing %carb increased serum creatinine (P = 0.001), increased urine creatinine excretion (P = 0.007), and decreased eGFRcreat by 1.5 mL/min/1.73 m2 (P = 0.02), reducing GI or both factors had no effect on serum creatinine, urine creatinine excretion, or eGFRcreat.

In general, the observed effects on kidney markers and GFR described above were confirmed when every possible dietary comparison was performed with the exception of creatinine and eGFRcreat, which demonstrated greater variability from changes in dietary factors (i.e. GI or %carb) (Additional file 1: Table S3).

We compared the diets’ effects on cystatin C, β2M, creatinine, eGFRcys, and eGFRcreat by strata of factors associated with CKD (Additional file 1: Table S1). There was no evidence of interactions by race, hypertension status, baseline triglycerides, BMI, and HOMA on any of the observed dietary effects. A sensitivity analysis utilizing feeding period 1 alone may be found in Additional file 1: Table S2. While the patterns of association were unaltered, our results were attenuated when we restricted our analysis to feeding period 1 only.

Finally, we specifically examined the mechanism by which GI and %carb affected eGFRcys. We found that the renal response to reducing GI was significantly associated with the renal response to reducing %carb (P < 0.001) (Additional file 1: Figure S2). Further, the effects of reducing GI or %carb on eGFRcys were only mildly attenuated with adjustment for potential mediators of kidney function, specifically, fasting glucose, insulin, triglycerides, uric acid, systolic blood pressure, and diastolic blood pressure (Table 4).

Table 4 Change in estimated glomerular filtration rate based on cystatin C adjusted for fasting glucose, fasting insulin, fasting triglycerides, uric acid, systolic blood pressure, diastolic blood pressure, and high sensitivity C-reactive protein
Full size table

Discussion

For the first time in a trial setting, we demonstrated that reducing GI, i.e. changing the type of carbohydrate, reduced markers of kidney function and increased glomerular filtration. Furthermore, this study confirmed prior observations that reducing %carb (i.e. increasing %protein/fat), increases kidney function. Moreover, an increase in eGFRcys from reducing GI tended to have a similar effect as that observed from reducing %carb, and these effects were additive, such that reducing both GI and %carb increased eGFRcys by 4.5 mL/min/1.73 m2. The effects persisted after adjustment for potential mediators of kidney function.

In our study, replacing carbohydrates with fat and protein increased GFR as estimated by cystatin C and β2M. These results are consistent with previous studies that test dietary effects on GFR [7]. Animal and human studies have indicated that dietary protein increases renal blood flow and glomerular filtration rates via increased intraglomerular pressures, leading to progressive glomerular sclerosis, particularly in the setting of decreased nephron mass [15–19]. These effects may be mediated by dietary effects on signaling molecules of the tubuloglomerular feedback system, responsible for arteriolar constriction [20–22]. It is also believed that dietary protein may increase neuronal nitric oxide synthase in the kidney cortex, which relaxes the afferent renal arteriole [23, 24]. Although fat was also higher in the low carbohydrate/high protein diet, there is much less evidence for a relationship between a higher fat diet and hyperfiltration [25], though some have hypothesized that such an association could be mediated through inflammation [26, 27].

This study is among the first to examine the effects of GI on GFR. GI, a metric of carbohydrate quality determined by measuring the amount of glucose released into circulation after consumption of a standard amount of carbohydrates [4], could influence kidney function. Reducing GI has been reported to decrease inflammation [28]. Furthermore, animal models have shown that a high GI diet contributes to glomerular degeneration [29]. Recently, an observational study of 2,600 adults reported a cross-sectional association between dietary GI and an estimated GFR <60 mL/min per 1.73 m2 (OR = 1.55 [1.07–2.26]; P-trend =0.01), but dietary GI was not associated with incident CKD over a 5-year follow-up period [6]. Contrary to the above studies and our a priori hypothesis, we found that a lower GI diet decreased kidney filtration markers and increased eGFRcys. While the exact mechanism for this observation is unknown, several animal models have reported an association between complex carbohydrates (i.e. low GI foods) and improved renal endothelial nitric oxide synthase [30, 31] or decreased leukocyte binding with glomerular endothelial cells [32]. Whether or not the increased GFR observed in conjunction with the low GI diets contributes adversely to clinical outcomes long-term remains to be determined.

Our study suggests that reducing GI or %carb share a common pathway for affecting eGFRcys (Additional file 1: Figure S2). These changes are independent of markers of plasma glucose homeostasis, endothelial function, and inflammation, implying that dietary carbohydrates may have a direct effect on glomerular filtration [33]. We suspect that these short-term increases in glomerular filtration from reducing GI and/or %carb, represent hyperfiltration, a maladaptive response to abnormal renal hemodynamics and a harbinger of kidney injury and kidney disease progression [34, 35]. The long-term impact of these short-term changes in eGFRcys on clinical outcomes is unknown. Novel markers of kidney injury in urine [36] may help elucidate the benefit or harm of dietary carbohydrates in future studies.

Cystatin C and β2-microglobulin (β2M) are low molecular mass plasma proteins that are alternative markers to creatinine for estimating GFR and predicting CKD progression [37–39]. Both cystatin C and β2M are generated at a constant rate by all nucleated cells, freely filtered by glomeruli, then reabsorbed and metabolized by the proximal tubule with minimal reentry to the bloodstream [40–43]. Serum creatinine reflects GFR, but as a byproduct of protein metabolism it is influenced by dietary protein [7] while cystatin C and β2M do not seem to be influenced by dietary protein [7, 16, 39]. Hence, these markers represent an attractive alternative to creatinine for quantifying GFR in feeding studies that involve changes in dietary protein [7].

In this trial, both cystatin C and β2M were affected by OMNICARB diets in a similar fashion, that is, lower %carb/higher protein or lower GI decreased plasma concentrations of both cystatin C and β2M. In contrast, creatinine did not decline with lower GI and further increased during the low %carb (higher protein)/high GI diet. In absence of measured GFR, this could be interpreted as either non-GFR effects on both cystatin C and β2M or non-GFR effects on creatinine. These observed effects had important ramifications on estimated GFR, which was significantly increased when estimated using cystatin C, but null or decreased when estimated using creatinine. Given the independent biosynthetic pathways of each of these three markers, it is more probable that non-GFR effects would influence one rather than two markers [10], that is in this case, creatinine versus both cystatin C and β2M. This underscores the value of non-traditional markers in better estimating changes in GFR [10, 44].

This study has limitations. The feeding periods were too brief to observe dietary effects on clinical events. Furthermore, the study did not include patients with diabetes or CKD, populations that might demonstrate greater changes in kidney function from alterations in dietary carbohydrates. Moreover, the diets were all DASH-like, healthful diets. It is possible that greater effects on kidney function would be observed if the reference diet was a typical American diet. Finally, it should be noted that by design reductions in %carb intake were coupled with increases in both fat and protein to keep overall caloric intake constant between diets. Thus, it would be inaccurate to conclude that reducing %carb alone increased kidney function. A better interpretation would be that replacing calories from carbohydrates with calories from protein and fat increased kidney function.

This study also has strengths, including being an ancillary of a randomized trial with a diverse population and high follow-up rates that obtained repeat measures of kidney function. Diets were tightly controlled and isocaloric, eliminating the potential effect of weight change on outcomes. Furthermore, we utilized alternative kidney markers instead of creatinine, which permitted more accurate assessments of diet-induced changes in GFR [7].

Conclusion

In conclusion, we found that both a low GI and low %carb (i.e. higher protein/fat) diets increased GFR, which possibly reflects hyperfiltration or risk of kidney injury. At this point it is unclear whether the increase in GFR caused by low GI diets would be associated with adverse clinical outcomes. This should be an important focus of future studies on GI and kidney function.

Abbreviations

OMNICARB:

The Effect of Amount and Type of Dietary Carbohydrates on Risk for Cardiovascular Heart Disease and Diabetes Study

CG:

The high carbohydrate, high glycemic index diet

cG:

The low carbohydrate, high glycemic index diet

Cg:

The high carbohydrate, low glycemic index diet

cg:

The low carbohydrate, low glycemic index diet

GFR:

Glomerular filtration rate

β2M:

β2-microglobulin

BMI:

Body mass index

LDL:

Low density lipoprotein

HDL:

High density lipoprotein

SBP:

Systolic blood pressure

DBP:

Diastolic blood pressure

DASH:

Dietary approaches to stop hypertension

GEE:

Generalized estimating equation

CI:

Confidence interval

References

  1. Levey AS, Adler S, Caggiula AW, England BK, Greene T, Hunsicker LG, Kusek JW, Rogers NL, Teschan PE. Effects of dietary protein restriction on the progression of advanced renal disease in the modification of diet in renal disease study. Am J Kidney Dis. 1996;27:652–63.

    Article  CAS  PubMed  Google Scholar 

  2. Pedrini MT, Levey AS, Lau J, Chalmers TC, Wang PH. The effect of dietary protein restriction on the progression of diabetic and nondiabetic renal diseases: a meta-analysis. Ann Intern Med. 1996;124:627–32.

    Article  CAS  PubMed  Google Scholar 

  3. Fouque D, Laville M, Boissel JP. Low protein diets for chronic kidney disease in non diabetic adults. Cochrane Database Syst Rev. 2006;2:CD001892.

  4. Jenkins DJ, Wolever TM, Taylor RH, Barker H, Fielden H, Baldwin JM, Bowling AC, Newman HC, Jenkins AL, Goff DV. Glycemic index of foods: a physiological basis for carbohydrate exchange. Am J Clin Nutr. 1981;34:362–6.

    CAS  PubMed  Google Scholar 

  5. A Report of the Panel on Macronutrients, Subcommittees on Upper Reference Levels of Nutrients and Interpretation and Uses of Dietary Reference Intakes, Standing Committee on the Scientific Evaluation of Dietary Reference Intakes. “6 Dietary Carbohydrates: Sugars and Starches.” Dietary Reference Intakes for Energy, Carbohydrate, Fiber, Fat, Fatty Acids, Cholesterol, Protein, and Amino Acids (Macronutrients). Washington, DC: The National Academies Press; 2005.

    Google Scholar 

  6. Gopinath B, Harris DC, Flood VM, Burlutsky G, Brand-Miller J, Mitchell P. Carbohydrate nutrition is associated with the 5-year incidence of chronic kidney disease. J Nutr. 2011;141:433–9.

    Article  CAS  PubMed  Google Scholar 

  7. Juraschek SP, Appel LJ, Anderson CAM, Miller 3rd ER. Effect of a high-protein diet on kidney function in healthy adults: results from the OmniHeart trial. Am J Kidney Dis. 2013;61:547–54.

    Article  CAS  PubMed  Google Scholar 

  8. Sacks FM, Carey VJ, Anderson CAM, Miller ER, Copeland T, Charleston J, Harshfield BJ, Laranjo N, McCarron P, Swain J, White K, Yee K, Appel LJ. Effects of high vs low glycemic index of dietary carbohydrate on cardiovascular disease risk factors and insulin sensitivity: the OmniCarb randomized clinical trial. JAMA. 2014;312:2531–41.

    Article  PubMed  PubMed Central  Google Scholar 

  9. Appel LJ, Moore TJ, Obarzanek E, Vollmer WM, Svetkey LP, Sacks FM, Bray GA, Vogt TM, Cutler JA, Windhauser MM, Lin PH, Karanja N. A clinical trial of the effects of dietary patterns on blood pressure. DASH Collaborative Research Group. N Engl J Med. 1997;336:1117–24.

    Article  CAS  PubMed  Google Scholar 

  10. Juraschek SP, Coresh J, Inker LA, Levey AS, Köttgen A, Foster MC, Astor BC, Eckfeldt JH, Selvin E. Comparison of Serum Concentrations of β-Trace Protein, β2-Microglobulin, Cystatin C, and Creatinine in the US Population. Clin J Am Soc Nephrol. 2013;8:584–92.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Inker LA, Schmid CH, Tighiouart H, Eckfeldt JH, Feldman HI, Greene T, Kusek JW, Manzi J, Van Lente F, Zhang YL, Coresh J, Levey AS. Estimating glomerular filtration rate from serum creatinine and cystatin C. N Engl J Med. 2012;367:20–9.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Levey AS, Stevens LA, Schmid CH, Zhang YL, Castro 3rd AF, Feldman HI, Kusek JW, Eggers P, Van Lente F, Greene T, Coresh J. A new equation to estimate glomerular filtration rate. Ann Intern Med. 2009;150:604–12.

    Article  PubMed  PubMed Central  Google Scholar 

  13. Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem. 1972;18:499–502.

    CAS  PubMed  Google Scholar 

  14. White H. A heteroskedasticity-consistent covariance matrix estimator and a direct test for heteroskedasticity. Econometrica. 1980;48:817–38.

    Article  Google Scholar 

  15. Brenner BM, Meyer TW, Hostetter TH. Dietary protein intake and the progressive nature of kidney disease: the role of hemodynamically mediated glomerular injury in the pathogenesis of progressive glomerular sclerosis in aging, renal ablation, and intrinsic renal disease. N Engl J Med. 1982;307:652–9.

    Article  CAS  PubMed  Google Scholar 

  16. Viberti G, Bognetti E, Wiseman MJ, Dodds R, Gross JL, Keen H. Effect of protein-restricted diet on renal response to a meat meal in humans. Am J Physiol. 1987;253(3 Pt 2):F388–393.

    CAS  PubMed  Google Scholar 

  17. Alavi FK, Zawada ET, Simmons JL. Renal hemodynamic and histological consequences of diets high in unsaturated fat, protein or sucrose in obese Zucker rats. Clin Nephrol. 1995;43:122–30.

    CAS  PubMed  Google Scholar 

  18. Schrijvers BF, Rasch R, Tilton RG, Flyvbjerg A. High protein-induced glomerular hypertrophy is vascular endothelial growth factor-dependent. Kidney Int. 2002;61:1600–4.

    Article  CAS  PubMed  Google Scholar 

  19. Meyer TW, Ichikawa I, Zatz R, Brenner BM. The renal hemodynamic response to amino acid infusion in the rat. Trans Assoc Am Physicians. 1983;96:76–83.

    CAS  PubMed  Google Scholar 

  20. Seney FDJ, Persson EG, Wright FS. Modification of tubuloglomerular feedback signal by dietary protein. Am J Physiol. 1987;252(1 Pt 2):F83–90.

    PubMed  Google Scholar 

  21. Seney FDJ, Wright FS. Dietary protein suppresses feedback control of glomerular filtration in rats. J Clin Invest. 1985;75:558–68.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Thomson SC, Vallon V, Blantz RC. Kidney function in early diabetes: the tubular hypothesis of glomerular filtration. Am J Physiol Renal Physiol. 2004;286:F8–15.

    Article  CAS  PubMed  Google Scholar 

  23. Yao B, Xu J, Qi Z, Harris RC, Zhang M-Z. Role of renal cortical cyclooxygenase-2 expression in hyperfiltration in rats with high-protein intake. Am J Physiol Renal Physiol. 2006;291:F368–374.

    Article  CAS  PubMed  Google Scholar 

  24. Tolins JP, Shultz PJ, Westberg G, Raij L. Renal hemodynamic effects of dietary protein in the rat: role of nitric oxide. J Lab Clin Med. 1995;125:228–36.

    CAS  PubMed  Google Scholar 

  25. Bouhanick B, Suraniti S, Berrut G, Bled F, Simard G, Lejeune JJ, Fressinaud P, Marre M. Relationship between fat intake and glomerular filtration rate in normotensive insulin-dependent diabetic patients. Diabete Metab. 1995;21:168–72.

    CAS  PubMed  Google Scholar 

  26. Cao J, Inoue K, Sodhi K, Puri N, Peterson SJ, Rezzani R, Abraham NG. High-fat diet exacerbates renal dysfunction in SHR: reversal by induction of HO-1-adiponectin axis. Obesity (Silver Spring). 2012;20:945–53.

    Article  CAS  Google Scholar 

  27. Lu J, Bankovic-Calic N, Ogborn M, Saboorian MH, Aukema HM. Detrimental effects of a high fat diet in early renal injury are ameliorated by fish oil in Han:SPRD-cy rats. J Nutr. 2003;133:180–6.

    CAS  PubMed  Google Scholar 

  28. Buyken AE, Flood V, Empson M, Rochtchina E, Barclay AW, Brand-Miller J, Mitchell P. Carbohydrate nutrition and inflammatory disease mortality in older adults. Am J Clin Nutr. 2010;92:634–43.

    Article  CAS  PubMed  Google Scholar 

  29. Adeyi AO, Idowu BA, Mafiana CF, Oluwalana SA, Ajayi OL, Akinloye OA. Rat model of food-induced non-obese-type 2 diabetes mellitus: comparative pathophysiology and histopathology. Int J Physiol Pathophysiol Pharmacol. 2012;4:51–8.

    PubMed  PubMed Central  Google Scholar 

  30. Roberts CK, Barnard RJ, Sindhu RK, Jurczak M, Ehdaie A, Vaziri ND. A high-fat, refined-carbohydrate diet induces endothelial dysfunction and oxidant/antioxidant imbalance and depresses NOS protein expression. J Appl Physiol. 2005;98:203–10.

    Article  CAS  PubMed  Google Scholar 

  31. Roberts CK, Vaziri ND, Sindhu RK, Barnard RJ. A high-fat, refined-carbohydrate diet affects renal NO synthase protein expression and salt sensitivity. J Appl Physiol. 2003;94:941–6.

    Article  CAS  PubMed  Google Scholar 

  32. Brady HR, Spertini O, Jimenez W, Brenner BM, Marsden PA, Tedder TF. Neutrophils, monocytes, and lymphocytes bind to cytokine-activated kidney glomerular endothelial cells through L-selectin (LAM-1) in vitro. J Immunol. 1992;149:2437–44.

    CAS  PubMed  Google Scholar 

  33. Schwingshackl L, Hoffmann G. Comparison of high vs. normal/low protein diets on renal function in subjects without chronic kidney disease: a systematic review and meta-analysis. PLoS One. 2014;9:e97656.

    Article  PubMed  PubMed Central  Google Scholar 

  34. Premaratne E, Macisaac RJ, Tsalamandris C, Panagiotopoulos S, Smith T, Jerums G. Renal hyperfiltration in type 2 diabetes: effect of age-related decline in glomerular filtration rate. Diabetologia. 2005;48:2486–93.

    Article  CAS  PubMed  Google Scholar 

  35. Brenner BM, Lawler EV, Mackenzie HS. The hyperfiltration theory: a paradigm shift in nephrology. Kidney Int. 1996;49:1774–7.

    Article  CAS  PubMed  Google Scholar 

  36. Miller ER, Juraschek SP, Anderson CA, Guallar E, Henoch-Ryugo K, Charleston J, Turban S, Bennett MR, Appel LJ. The effects of n-3 long-chain polyunsaturated fatty acid supplementation on biomarkers of kidney injury in adults with diabetes: results of the GO-FISH trial. Diabetes Care. 2013;36:1462–9.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  37. Aksun SA, Ozmen D, Ozmen B, Parildar Z, Mutaf I, Turgan N, Habif S, Kumanliogluc K, Bayindir O. Beta2-microglobulin and cystatin C in type 2 diabetes: assessment of diabetic nephropathy. Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association. 2004;112:195–200.

    Article  CAS  Google Scholar 

  38. Viberti GC, Keen H, Mackintosh D. Beta 2-microglobulinaemia: a sensitive index of diminishing renal function in diabetics. Br Med J (Clin Res Ed). 1981;282:95–8.

    Article  CAS  Google Scholar 

  39. Tangri N, Inker LA, Tighiouart H, Sorensen E, Menon V, Beck G, Shlipak M, Coresh J, Levey AS, Sarnak MJ. Filtration markers may have prognostic value independent of glomerular filtration rate. J Am Soc Nephrol. 2012;23:351–9.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  40. Vincent C, Revillard JP. Beta-2-microglobulin and HLA-related glycoproteins in human urine and serum. Contrib Nephrol. 1981;26:66–88.

    Article  CAS  PubMed  Google Scholar 

  41. Filler G, Bökenkamp A, Hofmann W, Le Bricon T, Martínez-Brú C, Grubb A. Cystatin C as a marker of GFR--history, indications, and future research. Clin Biochem. 2005;38:1–8.

    Article  CAS  PubMed  Google Scholar 

  42. Finney H, Newman DJ, Gruber W, Merle P, Price CP. Initial evaluation of cystatin C measurement by particle-enhanced immunonephelometry on the Behring nephelometer systems (BNA, BN II). Clin Chem. 1997;43(6 Pt 1):1016–22.

    CAS  PubMed  Google Scholar 

  43. Filler G, Priem F, Lepage N, Sinha P, Vollmer I, Clark H, Keely E, Matzinger M, Akbari A, Althaus H, Jung K. Beta-trace protein, cystatin C, beta(2)-microglobulin, and creatinine compared for detecting impaired glomerular filtration rates in children. Clin Chem. 2002;48:729–36.

    CAS  PubMed  Google Scholar 

  44. Rebholz CM, Grams ME, Matsushita K, Selvin E, Coresh J. Change in novel filtration markers and risk of ESRD. Am J Kidney Dis. 2014.

Download references

Acknowledgments

The following companies donated food: The Almond Board, International Tree Nut Council, Olivio Premium Products Inc, and The Peanut Institute. This trial is registered at clinicaltrials.gov, number: NCT00608049 (first registered January 23, 2008).

Funding

Funding for this study was provided through grants HL084568 and HL084568 from the National Institutes of Health. SPJ is supported by a NIH/NIDDK T32DK007732-20 Renal Disease Epidemiology Training Grant. ERM was supported by NIH/NHLBI R01HL095448-04.

Availability of data and materials

Data may be made available by contacting the corresponding author.

Authors’ contributions

SPJ analyzed data, wrote manuscript. ERM designed study, wrote manuscript. JC & LT participated in the design of the study. LA participated in the design of the study and provided critical edits. AC, CA, and DC provided critical edits. All authors read and approved the final manuscript.

Authors’ information

SPJ, LJA, DCC, LT, JC, ERM: The Johns Hopkins School of Medicine, The Johns Hopkins Bloomberg School of Public Health, and The Welch Center for Prevention, Epidemiology and Clinical Research, Johns Hopkins Medical Institutions, Baltimore MD

ARC: Geisinger Health System, Danville, PA

CAMA: University of California San Diego, San Diego, CA.

Competing interests

The authors have no conflicts of interest to report. Portions of this manuscript were presented as an oral presentation at the American Heart Association Epidemiology and Prevention, Lifestyle and Cardiometabolic Health Conference on March 5th, 2015.

Consent for publication

Not applicable.

Ethics approval and consent to participate

Institutional Review Boards at Harvard University and Johns Hopkins University approved the study protocol, and all participants provided written informed consent.

Author information

Authors and Affiliations

  1. The Johns Hopkins School of Medicine, The Johns Hopkins Bloomberg School of Public Health, and The Welch Center for Prevention, Epidemiology and Clinical Research, Johns Hopkins Medical Institutions, 2024 East Monument Street, Suite 1–500, Baltimore, MD, 21205, USA

    Stephen P. Juraschek, Lawrence J. Appel, Deidra C. Crews, Letitia Thomas, Jeanne Charleston & Edgar R. Miller III

  2. Geisinger Health System, Danville, PA, USA

    Alex R. Chang

  3. University of California San Diego, San Diego, CA, USA

    Cheryl A. M. Anderson

Authors
  1. Stephen P. Juraschek
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Alex R. Chang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lawrence J. Appel
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Cheryl A. M. Anderson
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Deidra C. Crews
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Letitia Thomas
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Jeanne Charleston
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Edgar R. Miller III
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Edgar R. Miller III.

Additional file

Additional file 1:

Figure S1. The effects (95% confidence intervals) of reducing glycemic index (GI), reducing the proportion of carbohydrates (%carb), or reducing both GI and %carb on: (A) creatinine (mg/dL) and (B) creatinine-based estimated glomerular filtration rate (eGFRcreat) (mL/min/1.73 m2) measured at the end of each feeding period. Figure S2. Scatter plot of the change in cystatin C-based estimated glomerular filtration rate (eGFRcys) (mL/min/1.73 m2) from reducing glycemic index (y-axis) versus reducing proportion of carbohydrate (x-axis). A linear regression line (dashed line) overlays the data (P-value of the coefficient was < 0.001). Table S1. Baseline change in glomerular filtration markers by subgroup (reference diet is the high glycemic index, high carbohydrate diet). Table S2. Between Diet Comparison Restricted to Visit 1 Only. Table S3. All Between Diet Comparisons. (DOCX 165 kb)

Rights and permissions

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Juraschek, S.P., Chang, A.R., Appel, L.J. et al. Effect of glycemic index and carbohydrate intake on kidney function in healthy adults. BMC Nephrol 17, 70 (2016). https://doi.org/10.1186/s12882-016-0288-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1186/s12882-016-0288-5

Keywords

BMC Nephrology

ISSN: 1471-2369

Contact us